芝麻素對於肥胖大鼠體脂肪蓄積與脂質代謝的影響

Effects of sesamin on fat accumulation and lipid metabolism in Sprague-Dawley rats

郭倍秀(Pai-Hsiu Kuo)

指導教授 ： 簡怡雯

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2008年)

https://doi.org/10.6831/TMU.2008.00056

摘要

芝麻中含量最多的木質酚類--芝麻素，以可促進體內脂肪酸氧化和減少脂肪酸生成而受到注目。本實驗中，採用雄性Sprague-dawley大鼠，經一週適應期後分為正常體重組(N)與肥胖組(F)各30隻，N組給予AIN93G飲食，F組給予45%熱量來自油脂的高脂飲食，四週後依體重將N組與F組各分為三組並添加不同劑量的芝麻素當作實驗飲食：N0與F0組不添加芝麻素、N1與F1添加0.1%芝麻素、N5與F5組添加0.5%芝麻素於飲食中。每週測量其體重變化及攝食狀況，給予四週實驗飲食後予以犧牲。結果指出N5組中，其血漿中三酸甘油酯(TG)、總膽固醇(TC)與低密度膽固醇(LDL-C)濃度都顯著下降，其肝臟中fatty acid synthase(FAS)活性顯著下降、carnitine palmitoyltransferase(CPT)與acetyl CoA oxidase(ACO)活性具有增加的趨勢，並可減少相對副腎脂肪和相對總脂肪的重量，但對體重增加情況沒有改變。在F5組結果指出，血漿中TG、LDL-C、游離脂肪酸濃度顯著下降、高密度膽固醇濃度顯著增加，其肝臟中FAS、Acyl CoA carboxylase活性顯著減少、CPT、ACO活性顯著增加，並可增加胰島素敏感性並可減少副睪和相對副睪脂肪、副腎和相對副腎脂肪及總脂肪和相對總脂肪的堆積情況，並可達到減輕體重的目的。

關鍵字

肥胖 ； 芝麻素 ； 體脂蓄積 ； 脂質代謝 ； 胰島素敏感性

並列摘要

Sesamin, a major lignan in sesame seeds and oil, has been known to increase β-oxidation and reduce lipogenesis. In this study, 30 sprague-dawley rats were fed with pure AIN-93G diet (standard diet) and 30 rats were fed with high fat diet (modified from AIN-93G containing 45% calories from soybean oil). After 4 weeks to induce obesity, rats were assigned to 6 groups: N0 (standard diet without sesamin), N1 (standard diet with 0.1% sesamin), N5 (standard diet with 0.5% sesamin), F0 (high fat diet without sesamin), F1 (high fat diet with 0.1% sesamin), F5 (high fat diet with 0.5% sesamin). The rats were weighted weekly and sacrificed after 4 weeks of experiment. Results found that there were significantly reducing in body weight, epididymal adipose tissue and adipocyte size in group F5. Supplement with sesamin led to significantly increase serum HDL-C and decrease plasma triglyceride, LDL-C, non-esterified fatty acid. Insulin sensitivity had also been improving in F5 group. Besides, there were significantly reducing in liver triglyceride and total cholesterol concentration. The enzyme activities of fatty acid synthase and acetyl CoA carboxylase were significantly decreasing in group F5 and acyl CoA oxidase, carnitine palmitoyltransferase were significantly increasing in group F1 and F5. Therefore, we concluded that sesamin can improve lipid metabolism and prevent the accumulation of white adipose tissue.

並列關鍵字

obesity ； sesamin ； fat accumulation ； lipid metabolism ； insulin sensitivity

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