利用分子基因造影技術研究樟芝防治癌症之 臨床前療效評估及其重要機轉與特色-癌症逆轉

Utilization of molecular imaging in monitoring and evaluating pre-clinical effects of Taiwanofungus camphoratus on cancer treatment and its potential in cancer reversion

王瑋婷(Wei-Ting Wang)

指導教授 ： 鄧文炳

臺北醫學大學/口腔醫學院/生物醫學材料研究所/碩士(2007年)

摘要

Tawanofungus camphoratus俗名稱為牛樟芝，為台灣特有的藥用真菌。本研究之目的為探討固態栽培樟芝酒精粗萃物中具有癌症逆轉(Cancer reversion)的特性並能抑制腫瘤細胞生長，再結合分子造影系統(Molecular imaging system)，即時觀察生物活體內細胞分子層次之變化。分別以人類肺癌細胞(H441GL cells)及子宮頸癌細胞(HeLa cells)作為篩選分析之細胞株。首先，以固態栽培樟芝酒精粗萃物作用於人類肺癌細胞及子宮頸癌細48小時，初步得到其IC50分別為100?慊/mL及300 ?慊/mL，樟芝酒精粗萃物具有明顯抑制腫瘤細胞生長能力。第一部份實驗分析樟芝酒精粗萃物調控H441GL肺癌細胞之反應，結果顯示可使H441GL肺癌細胞週期停滯於G0/G1時期，並隨著劑量增加而抑制H441GL肺癌細胞之遷移性(Cell migration)，但對細胞毒殺不走凋亡路徑(Apoptosis)，而是走壞死路徑(Necrosis)。在動物實驗方面，每三天餵食500?慊樟芝酒精粗萃物的確具有抑制H441GL肺癌腫瘤生長之效果。第二部份實驗，樟芝酒精粗萃物亦能抑制HeLa子宮頸癌細胞內鹼性磷酸酵素(Alkaline phosphates)表現，並具有癌症逆轉之特性，抑制E7致癌蛋白和提升p53、pRB抑癌基因表現。研究顯示樟芝酒精粗萃物對肺癌細胞株(H441GL cell/p53 mutant)和子宮頸癌細胞株(HeLa cell/p53 wild type)之p53蛋白表現量無顯著影響，顯示樟芝之酒精粗萃物在調控p53蛋白作用機轉中無明顯之影響能力，於動物實驗中樟芝確實具有抑制HeLa子宮頸癌腫瘤細胞生長之效果，故應用分子造影能提供快速與有效地評估樟芝酒精萃物之抑制腫瘤療效。

關鍵字

樟芝 ； 分子造影系統 ； 癌症逆轉 ； 鹼性磷酸酵素

並列摘要

The fruiting body of Taiwanofungus camphoratus is well known in Taiwan as
“niu-chang chih” or “jang-jy”. The objective of this research is to identify the bioactive compounds in Taiwanofungus camphoratus’s ethanol extract from solid-state growen with cancer reversion and anti-tumor activities. This non-invasive molecular imaging is useful in assessing tumor volume in vivo. By treating H441GL cells ( human non-small lungs cancer cells ) and HeLa cells ( human cervical cancer cells ) with ethanol extracts of Taiwanofungus camphoratus for 48 hrs. The ethanol extract was found to possess the highest anti-tumor activities with the IC50 values of 100?慊/ml and 300 ?慊/ml, respectively. Data from flow cytometry analysis indicated that Taiwanofungus camphoratus-mediated antiproliferation effect related to G0/G1 phase arrest on H441 cells. Moreover, this Taiwanofungus camphoratus –induced G0/G1 cell cycle arrest was associated with decrease the expression levels of cyclin A and increase in cyclin D2、cyclinE proteins . Dose-dependent treatment of H441GL cells with Taiwanofungus camphorate resulted in the inhibition of cell migration and commitment to cell necrosis. Taiwanofungus camphoratus induced tumorigenic HeLa cells to revert back to a normal-like phenotypic state (cancer reversion), such as the loss of alkaline phosphatase expression. This reversion was associated with the down- regulation of E7 oncoproteins and subsequent reactivation of p53/pRB gene. In vivo animal tumorigenicity studies were demonstrated that Taiwanofungus camphorates was effective inhibited tumor grow. These results suggest that Taiwanofungus camphorates may be used as a potent agent for cancer therapy.

並列關鍵字

Cancer reversion ； Alkaline phosphates ； Molecular imaging system ； Taiwanofungus camphoratus ； E6/E7 oncoproteins ； p53 tumor suppressor ； cell cycle ； necrosis ； cell migration

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