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  • 學位論文

紅甘藷葉的攝取對健康男性運動後體內壓力指標及發炎反應之影響

Effect of Purple Sweet Potato Leaves Consumption on the Exercise-induced Stress Markers and Inflammation in Healthy Male

指導教授 : 胡雪萍
共同指導教授 : 劉珍芳

摘要


本研究的目的是先利用 HPLC 分析出紅甘藷葉中主要的植物化學物質的種類及含量;並探討給予紅甘藷葉飲食,對健康男性運動後體內壓力指標及發炎反應之影響。實驗共分三個部份,第一部份:將紅甘藷葉進行萃取,利用一般呈色法及 HPLC 配合 PDA 檢測系統,分析其類胡蘿蔔素 (carotenoids)、酚酸 (phenolic acids)、類黃酮 (flavonoids) 及花青素 (anthocyanins) 的種類及含量。第二部份:人體試驗採隨機交叉設計,招募健康成年男性為受試者。實驗為期共五週,第一週為適應期,之後進行兩階段分別為期 7 天的飲食介入期 (紅甘藷葉 vs. 控制/低多酚飲食),且間隔 14 天的排空期。分別於兩階段飲食介入期結束後,受試者進行 70% VO2max 的運動達 1 小時,並於運動前及結束後第 0、1 及 3 小時 (Post-E0, Post-E1h 及 Post-E3h) 抽取受試者血液樣本以進行氧化壓力指標、IL-6 及熱休克蛋白表現分析。第三部份:人體試驗設計同第一部份,並將飲食介入期延長為 14 天;運動後採集血液的時間點拉長至 48 小時。於兩階段飲食介入期間,會收集實驗飲食樣本以進行植物化學物質含量分析;受試者血液及尿液樣本以進行四大壓力指標、促/抗發炎細胞激素濃度及 NF-κB 蛋白表現分析。綜合三部份研究結果顯示,紅甘藷葉中含有 59.3 ± 3.05 mg gallic acid equivalent (GAE)/g dry weight (DW) 的總多酚類濃度、10.6 ± 0.69 mg quercetin equivalent (QCE)/g DW 的總類黃酮濃度及 1.34 ± 0.04 mg/g DW 的總花青素濃度;經 HPLC 分析後發現含有較高含量屬於類胡蘿蔔素的 lutein 及 β-carotene;屬酚酸的 caffeic acid;屬類黃酮的 quercetin 及屬花青素的 cyanidin。而與控制飲食相比,紅甘藷葉飲食也含有較高含量的總多酚類、總類黃酮及總花青素濃度 (2.85 vs. 0.63 mg GAE/g DW; 0.36 vs. 0.01 mg QCE/g DW; 0.03 vs 0 mg/g DW, p<0.0001)。因此受試者攝食紅甘藷葉飲食後,血漿及尿液中總多酚類濃度會顯著增加;血中總抗氧化能力 (如 FRAP) 也顯著上升;而受試者進行 1 小時的中強度運動後,血漿中四大壓力指標及PBMC中 NF-κB 蛋白表現量皆呈現顯著的飲食介入效應:氧化壓力指標 (TBARS & PC)、機械性壓力指標 (CK)、代謝性壓力指標 (cortisol) 及熱壓力指標 (HSP72);然而對於促/抗發炎細胞激素則無顯著影響。結論:長期攝取富含植物化學物質的紅甘藷葉飲食,可增加體內的抗氧化力以抵禦因運動所誘導生成的各種壓力傷害,然而對於發炎反應則無延緩的效果。

並列摘要


PURPOSES: This study was to identify and quantify the phytochemical contents of purple sweet potato leaves (PSPLs) by high-performance liquid chromatography (HPLC) and evaluate the effects of PSPLs diet consumption on the time course of exercise-induced stress markers and inflammation. METHODS: The study was distributed into three parts. Part 1 Study: The carotenoid, flavonoid, and anthocyanin contents of PSPLs extract were measured by spectrophotometry and HPLC coupled to photodiode array (PDA) detection. Part 2 Study: A crossover design was applied, subjects participated in a two-step dietary intervention period. Each subject was given a high- (PSPLs) or low-polyphenol (control) diet for 7 days with a 2 weeks washout period. After each dietary intervention period, all subjects performed 1 hour of treadmill running at a speed corresponding to 70% of each subject’s individual maximal oxygen uptake (VO2max). Blood samples were taken before exercise and at 0, 1, and 3 hours (Post-E0, Post-E1h 及 Post-E3h) after exercise. Part 3 Study: This study followed the design used by Study 1. We extended the dietary intervention for 14 days and the blood collection required up to 48 hours after exercise. RESULTS: The total polyphenol, flavonoid and anthocyanin contents of PSPLs were 59.3 ± 3.05 mg GAE/g DW, 10.6 ± 0.69 mg QCE/g DW, and 1.34 ± 0.04 mg/g DW, respectively. Very high concentrations of lutein, β-carotene, caffeic acid, quercetin, and cyanidin were found in PSPLs. There is a significant difference between two diets in total polyphenol, flavonoid and anthocyanin contents (2.85 vs. 0.63 mg GAE/g DW; 0.36 vs. 0.01 mg QCE/g DW; 0.03 vs 0 mg/g DW, p<0.0001). PSPLs consumption significantly increased plasma and urine total polyphenols concentration and total antioxidant power (ferric reducing ability of plasma; FRAP). The pattern of change between two dietary interventions was significant for stress markers (oxidative stress: TBARS and PC, intervention main effect, p=0.013 and 0.031; mechanic stress: CK, p=0.049; metabolic stress: cortisol, p=0.014 and thermal stress: HSP72, p=0.002) and NF-κB protein expression. However, there was no significant difference between two diets in pro-/anti- inflammatory cytokines. CONCLUSION: Consumption of PSPLs diet, a meal rich in phytochemical contents, could offer protection against the stress damage caused by exercise, but fail to attenuate inflammation.

參考文獻


林筱涵 (2009) 含杏仁果及紅甘藷葉之飲食介入對力竭性耐力運動後抗氧化及運動能力之影響。台北醫學大學保健營養學系碩士論文。
Chang WH, Chen CM, Hu SP, Kan NW, Chiu CC, and Liu JF (2007a) Effect of purple sweet potato leaf consumption on the modulation of the antioxidative status in basketball players during training. Asia Pac J Clin Nutr 16:455-461.
Chang WH, Chen CM, Hu SP, Kan NW, Chiu CC, and Liu JF (2007b) Effect of purple sweet potato leaves consumption on the modulation of the immune response in basketball players during the training period. Asia Pac J Clin Nutr 16:609-615.
Chiu HL and Fan MJ (1998) Anthocyanin and flower color expression. J Chinese Soc Hort Sci 44:102-115.
鄭統隆、施怡如、曾東海、賴永昌、吳明哲 (2008) 甘藷花青素與多酚含量之研究。台灣農業研究 57:33-48。

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