- 學位論文
利用磁振造影評估以重建膠原蛋白模板與高含量血小板血漿植入後膝關節半月板軟骨之再生─兔子動物實驗模型
Magnetic Resonance Imaging Correlated with Histological Evaluation of Meniscal Regeneration Using Collagen-Platelet Rich Plasma in Knee Joints of Rabbits
摘要
中文題目：利用磁振造影評估以重建膠原蛋白模板與高含量血小板血漿植入後膝關節半月板軟骨之再生-兔子動物實驗模型 以膠原蛋白模板促進膝關節半月板之再生已成功研發，另外高含量血小板血漿PRP(platlet-rich plasma)內含生長因子的生物材料亦已應用於臨床，本實驗為前測試驗，其目的是結合上述兩種材料特性，經由動物實驗以手術植入受損之半月板，利用非侵入性磁振造影(MRI)與組織切片對照觀察半月板的再生過程。 本實驗包含8隻紐西蘭母白兔(約3個月大小，重約3公斤)，分為3組： A組：半月板切除術合併高含量血小板血漿PRP和重建膠原蛋白模板之混合物種植(共5隻兔、右膝) B組：半月板切除術(共3隻兔、右膝) C組：正常半月板(利用A組兩隻兔之左膝) MRI使用1.5-T儀及3-in之表面線圖，成像技術包括T1-, PD-, T2-加強影像及注射對比劑(Gadopentate dimeglumine)後的T1-加強影像。我們將固定以右膝關節的外側半月板中央區軟骨做為MRI影像，於手術前(0週、A1-5、B1-3)，術後第4(A1、B1)、8(A2、B2)、12週(A3、B3)及24週(A4、A5)為MRI觀察期，隨即進行撲殺做組織切片。對照切片顯示組織之再生過程發現：MRI影像呈現高訊號表示組織再生不良，切片顯示有纖維母細胞增生現象。相反地，MRI影像呈現低訊號表示組織再生良好，切片結果顯示與原組織呈現相同染色。本實驗初步發現，手術後植入混合膠原蛋白與PRP生物材料，三個月後，其組織再生情況明顯優於未植入上述生物材料者，但再生組織之重塑(tissue remodeling)需要至6個月或更長的時間觀察。
並列摘要
Magnetic Resonance Imaging Correlated with Histological Evaluation of Meniscal Regeneration Using Collagen-Platelet Rich Plasma in Knee Joints of Rabbits Previously, our laboratory has developed a collagen template for disc regeneration of knee meniscus and TMJ. Recently, biomaterial PRP (Platelet-Rich Plasma) has been applied in clinics. This pilot study attempts to employ both PRP and collagen templates to stimulate regeneration of the meniscal tissue of the knee in rabbit model by using MR imaging assessment and correlates the MR findings with histology. A total of 8 adult New Zealand female rabbits (age: 3 months old; weight: 3kg) were recruited and divided into 3groups: (Group A) partial meniscectomy with PRP and collagen implantation with follow-up at 4 weeks(A1), 8 wks(A2), 12 wks(A3), and 24 wks(A4, A5), and (Group B) those rabbits with partial meniscectomy only and follow-up at 4 weeks(B1), 8 wks(B2) and 12 wks(B3), Group C was a normal knee. (using 2 of the rabbits’ left knee in group A). MR imaging was performed on a 1.5-T magnet (Horizon LX, G.E.) by using sagittal T1-weighted, PDI, T2W, and postcontrast (gadopentetate dimeglumine) T1W images. The right knee was consistently to be acted as the experimental side for MR imaging and histology. MR imaging findings of higher signal with formless structure of the meniscal tissue indicated relative poor regeneration, which correlates well with histology. The histology revealed fibroblasts proliferation. On the contrary, regenerative meniscal tissue exhibits lower signal intensity relative to the parent meniscus. Our preliminary data concluded that regeneration of meniscal tissue after PRP and collagen implantation in 3 months, and further follow-up to 6 months is needed to observe entire remodeling process of regeneration. MR imaging of low signal intensity of the regenerative meniscal tissue correlates well with collagen-rich native tissue.
參考文獻
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