利用液液兩相分配式生物反應器降解高鹽份中之酚溶液

Biodegradation of Phenol in Saline and Acidic Solution Using a Liquid-Liquid Two-Phase Partitioning Bioreactor.

曾國瑞(Kuo-Jui Tseng)

指導教授 ： 莊瑞鑫

元智大學/工程學院/化學工程與材料科學學系/碩士(2008年)

https://doi.org/10.6838/YZU.2008.00122

摘要

工業廢水中含有許多酚化合物，如不加以處理而直接排放，會對整個生態環境造成嚴重的汙染，所以本研究以Pseudomonas putida採用液液兩相分配式生物反應器降解含有酚之高鹽及強酸水溶液。
首先利用懸浮系統，對於不同基質濃度降解之影響來探討其動力學，而Pseudomonas putida對酚有抑制現象，故採用Haldane’s model去模擬細菌生長與降解，其得到之動力參數，經過曲線擬合(Curve fitting)後的最小標準平方差皆在0.93以上。而於液液兩相分配式生物反應器實驗中，藉由不同實驗條件，如初始酚濃度效應、鹽濃度效應、pH效應、攪拌速率效應及曝氣速率效應等，探討含酚之高鹽及強酸廢水在此系統中之生物降解效率。
由實驗結果得知，以煤油為有機溶劑，最高可處理含酚廢水之上限濃度為3000 mg/L，其中當操作條件在酚濃度1200 mg/L、添加10 % NaCl、轉速50 rpm、廢水端pH值使用(1M)鹽酸調至pH 3.0、生物端使用磷酸緩衝液調初使pH值至pH 7.2及曝氣速率0.1 vvm時，皆具有最佳移除效率。
最後，結合質傳理論與生物動力學的結果，預測細菌於液液兩相分配式生物反應器中降解酚，在總基質濃度為1200 mg/L以下時，酚降解時間之模擬曲線，與實驗點相當符合。

關鍵字

液液兩相分配式生物反應器 ； 生物降解

並列摘要

Phenolic compounds are often encountered in industrial effluents. The discharge of wastewater without proper treatment poses serious environmental problems. Thus, using a liquid-liquid two-phase partitioning bioreactor to degrade phenol in saline and acidic solution by Pseudomonas putida was investigated.
In free suspension system, the kinetic parameters were obtained from each concentration of substrate in biodegradation. The kinetic studies have shown that phenol would inhibit the growth of Pseudomonas putida. It was used Haldane’s model to simulate the cell growth and the phenol biodegradation. After curve fitting by MATLAB doftlab software, all of the correlation coefficients were above 0.93.
In liquid-liquid two-phase partitioning bioreactor module, it was found the optimal biodegradation efficiencies by different operation conditions, such as initial phenol concentration effect, NaCl concentration effect, pH effect, agitation rate effect and aeration rate effect.
From the experimental results, the maximum concentration of phenol biodegradation is less than 3000 mg/L when kerosene is used as solvent. In addition, it was obtained the optimal degradation efficiencies when operation conditions were at phenol concentration of 1200 mg/L and NaCl concentration of 10 % with 1M HCl to adjust pH to 3.0 in wastewater side; agitation rate of 50 rpm, aeration rate of 0.1 vvm, and addition of phosphate buffer to adjust pH to 7.2 in cell side.
At last, the system was combined kinetic results and mass transfer theory to predict the dynamic of substrate degradation and cell growth in liquid-liquid two-phase partitioning bioreactor. In comparing between the model line and the experimental data, when the substrate concentrations were both less than 1200 mg/L, phenol were biodegraded completely in the approximate duration.

並列關鍵字

Liquid-liquid two-phase partitioning bioreactor ； biodegradation

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