24小時高糖環境下之臍靜脈內皮細胞基因表現變化

Analysis of gene expression changes in huvecs under 24-hour high glucose exposure

賴宗賢(Tzung-Shian Lai)

指導教授 ： 翁資雅

元智大學/資訊學院/資訊工程學系/碩士(2013年)

https://doi.org/10.6838/YZU.2013.00289

摘要

糖尿病是在大多數國家，最常見的慢性疾病之一，受到糖尿病影響的人不斷的增加，隨著改變生活方式活動力減少導致肥胖，高血糖的破壞性影響，可能導致多種併發症，包含糖尿病冠狀動脈疾病、外周動脈疾病、腎病、神經病變、視網膜病變。根據世界衛生組織(WHO)和美國糖尿病協會，估計在2030年將會有3億4700萬人患有糖尿病。
高血糖誘導活性氧和血管內皮細胞凋亡，在高糖誘導血管內皮細胞凋亡的分子機制上目前得知的甚少，高糖對於臍靜脈內皮細胞的培養是學習與糖尿病血管併發症的方法，研究臍帶血在24小時高糖環境下基因表現量的變化，以特定時間點觀察細胞開始凋亡的表現，希望得到確定的候選基因，並調控這些基因表達的變化機制，幫助預防糖尿病引起的血管併發症。

關鍵字

臍帶血 ； 高糖 ； 第二型糖尿病 ； 血管併發症

並列摘要

Diabetes mellitus is one of the most common chronic diseases in most countries, and the number of affected people continues to increase, as changing lifestyle leads to reduced physical activity and increased obesity. The damaging effects of hyperglycemia can cause a variety of complications, including diabetic coronary artery disease, peripheral arterial disease, nephropathy, neuropathy, and retinopathy. According to the World Health Organization (WHO) and the American Diabetes Association, it is estimated that in 2030 there will be 347 million people affected with diabetes in the world.
High glucose can induce reactive oxygen species and apoptosis in endothelial cells. Little is known about the molecular mechanisms in high glucose induced endothelial cell apoptosis. High glucose treatment on human umbilical vein endothelial cells is the general approach for studying vascular complications associated with diabetes. More and more research groups are also focusing on assessing the gene expression changes under high glucose exposure. This study attempts to investigate the gene expression changes in HUVECs under 24 hours of high glucose treatment. This is the particular time point before cells start to show phenotypes of cell death. Understanding the mechanisms regulating these gene expression changes, we hope to identify candidate genes that can help us develop better prevention strategies for vascular complications associated with diabetes.

並列關鍵字

huvecs ； high glucose ； T2D ； Vascular complications

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