台灣原住民高三酸甘油脂血症與脂蛋白元C-III /脂蛋白解脂酶的基因多型性之相關性探討

Association between hypertriglyceridemia and apolipoprotien C-III/lipoprotein lipase gene polymorphism in Taiwan Aborigines

劉怡麟(I- Lin Liu)

指導教授 ： 葛應欽 ； 黃孟娟

高雄醫學大學/醫學院/醫學研究所/碩士(2003年)

摘要

[摘要]
背景－高三酸甘油脂血症(Hypertriglyceridemia，HTG)是一種原因複雜的代謝性異常疾病。我們評估環境因子與脂蛋白C-III(apolipoprotien C-III，APOC III) Sst І以及脂蛋白脂解脂(lipoprotein lipase，LPL) Hind III基因多型性對台灣原住民HTG之相關性。
方法－本研究利用橫斷性研究，將250位台灣南部原住民(包括排灣族與阿美族)分為HTG-90人以及NTG-正常TG組當作健康對照。HTG的定義為TG>150mg/dl(NCEP，2001)。研究資料收集是由已訓練之訪員利用結構式問卷。將萃取的DNA，利用聚合鏈反應（ Polymerase Chain Reaction, PCR）分析再以RFLP（restriction fragment length polymorphism）方法分析APOC III(Sst І)以及LPL(Hind III)基因的多型性。
結果－APOC III(Sst І)主要對偶基因(S1)以及稀少對偶基因(S2)，在HTG組的S1和S2對偶基因頻率為66.1﹪和33.9﹪，而在NTG組的S1和S2對偶基因頻率為73.6﹪和26.4﹪(P<0.1)。但是獨立分析女性樣本時，S2對偶基因頻率在HTG組有顯著的偏高(P<0.03)。LPL(Hind III) 對偶基因型，在HTG組的H-和H+對偶基因頻率為15.7﹪和84.3﹪而NTG組的H-和H+對偶基因頻率為21.1﹪和78.9﹪，並無顯著差異。經由多變項邏輯迴歸分析，發現阿美族、嚼檳榔習慣、攝取較多澱粉類食物以及偏高ApoC3含量與HTG之危險性有顯著相關。進一步，我們發現ApoC3含量在不同APOC III基因多型性S1S1、S1S2、S2S2會有劑量效應而增加的趨勢。
結論－本研究的分析結果得知，台灣原住民族群其APOC III(Sst І)基因多型性可能與HTG之危險性有相關。

關鍵字

台灣原住民 ； 高三酸甘油脂血症 ； 脂蛋白解脂酶脂蛋白元C-III

並列摘要

Abstract
Background － Hypertriglyceridemia(HTG) is a heterogeneous metabolic disorder. Effects of environmental factors and two genetic polymorphisms, an SstI polymorphism of Apolipoprotein C III gene and a Hind III polymorphism of lipoprotein lipase gene, on risk of HTG were analyzed in 250 Taiwan aborigines with or without HTG.
Methods － This is a cross-sectional study of 250 southern Taiwan aborigines, which composed Paiwan tribe and Amis tribe, with 90 cases in the HTG group and 160 with normal serum triglycerides (NTG) recruited from community Health examinations. HTG is defined as > 150 mg/dl according to report by National Cholesterol Education Program (2001). Demographic data and dietary habits were collected by trained interviewers using structured questionaires. Polymerase chain reaction — restriction fragment length polymorphism (PCR — RFLP) was performed to define gene polymorphisms.
Results － The respective SstI major allele (S1) and minor allele (S2) frequencies were 66.1﹪and 33.9﹪in HTG group and 73.6% and 26.4 % in NTG group (p<0.1). As analyzed exclusively in female subjects, frequencies of S2 allele was significantly higher (p<0.03) in HTG. The frequencies of the HindIII major allele (H+) and minor allele (H-) were similar between HTG (H+ 84.3﹪; H-:15.7﹪) and NTG (H+ 78.9%; H-:21.1%). Multiple logistic regression analysis reveals that Amis tribe, betel-chewing, starchy food, and plasma ApoC3concentrations were independently associated with risk of HTG. Furthermore, ApoC3 concentrations were increased (p<0.01) in a dose response manner among subjects with APOCIII s1s1, s1s2 and s2s2 genotypes accordingly.
Conclusions－ In conclusion, our analyses suggest that molecular variants of APOC III(Sst І) polymorphism may be associated risk of HTG in Taiwan Aborigines.

並列關鍵字

Taiwan Aborigines ； Hypertriglyceridemia ； lipoprotein lipase ； apolipoprotien C-III

參考文獻

第七章 參考文獻（References）

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