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  • 學位論文

台灣特有種植物長葉黃芩的高效能液相層析指紋圖譜分析及品質評價研究

Utilizing HPLC Fingerprint to Analyze the Quality of Scutellaria hsiehii, an Endemic Plant in Taiwan

指導教授 : 顏銘宏

摘要


黃芩 (Scutellariae Radix) 為台灣常用之傳統中藥材,近年來的藥理學研究證實其活性成分具有抗發炎、抗菌、抗病毒、抗癌以及抗氧化等等作用。過去研究指出,台灣的6種黃芩屬植物,南臺灣黃芩 (S. austrotaiwanensis)、半枝蓮 (S. barbata)、印度黃芩 (S. indica)、布烈氏黃芩 (S. playfairi)、臺灣黃芩 (S. taiwanensis) 以及田代氏黃芩 (S. tashiroi) 大多包含黃芩苷 (baicalin)、黃芩素 (baicalein) 以及漢黃芩素 (wogonin) 等3種指標成分。 台灣本土的黃芩產量極少,市售藥材多需仰賴進口,有鑑於市售黃芩藥材的基原未明、品質參差不齊,應尋求可應用之其他黃芩屬植物,檢測其有效成分含量,以評估取代市售黃芩藥材之可能性。本研究以近年發現的台灣特有種植物--長葉黃芩 (S. hsiehii) 為主,利用高效能液相層析 (high-performance liquid chromatography; HPLC) 進行黃芩苷、漢黃芩苷 (wogonoside)、黃芩素以及漢黃芩素等4種指標成分之定量分析,建立其指紋圖譜 (fingerprint),並與其他6種台灣黃芩屬植物及市售黃芩藥材相比較,以探討台灣黃芩屬植物的利用價值。 實驗結果顯示新品種長葉黃芩含有黃芩苷、漢黃芩苷、黃芩素以及漢黃芩素等4種指標成分,三批長葉黃芩檢品之黃芩苷含量平均值為4.50±0.17 mg/g;漢黃芩苷含量平均值為3.04±0.09 mg/g;黃芩素含量平均值為0.46±0.02 mg/g;漢黃芩素含量平均值為0.48±0.03 mg/g。 HPLC指紋圖譜之分析結果顯示,台灣7種黃芩屬植物有11支共有吸收峰,7種黃芩屬植物當中,僅有台灣黃芩未檢出漢黃芩素,其餘6種黃芩屬植物之HPLC圖譜均有出現黃芩苷、黃芩素、漢黃芩苷以及漢黃芩素之吸收峰。此外,7種台灣黃芩屬植物的N強峰數分別為9~11支不等,其佔總吸收峰積分面積之比例介於67.56~81.64 %之間,以田代氏黃芩 (81.64 %) 為最高,其次是南台灣黃芩 (79.87 %) 和長葉黃芩 (78.88 %)。 台灣市售黃芩藥材與7種黃芩屬植物有5支共有吸收峰,包含黃芩苷、黃芩素以及漢黃芩苷等3個指標成分。在黃芩苷含量方面,長葉黃芩 (4.50±0.17 mg/g)、田代氏黃芩 (4.83±1.00 mg/g) 與南臺灣黃芩 (4.60±0.79 mg/g) 的黃芩苷含量並沒有比品質較差之市售黃芩藥材-2 (4.07±1.83 mg/g) 少;在漢黃芩苷含量方面,長葉黃芩 (3.04±0.09 mg/g) 及田代氏黃芩 (3.16±0.78 mg/g) 的含量與品質優良之市售黃芩藥材-1 (2.96±1.23 mg/g) 不相上下。此研究結果顯示,對於取代市售黃芩藥材而言,新品種長葉黃芩、田代氏黃芩以及南台灣黃芩為值得開發之品種。

並列摘要


The root of Scutellaria baicalensis, known as Scutellariae Radix, is one of the most widely used traditional Chinese medicine in Taiwan. Besides, the effect of anti-inflammatory, antibacterial, antiviral, anticancer and antioxidation activities has been confirmed by many researches. Previous study has demonstrated that 6 species of Scutellaria plants in Taiwan, which were S. austrotaiwanensis, S. barbata, S. indica, S. playfairi, S. taiwanensis and S. tashiroi, contain three main bioactive components, baicalin, baicalein and wogonin. Due to the low yield of Scutellariae Radix in Taiwan, the commercial products are mainly imported from mainland China. The origins of the imported products can’t be determined and the quality control are difficult to implement. Therefore, we attempted to establish the HPLC fingerprint of S. hsiehii and measured the content of baicalin, wogonoside, baicalein and wogonin of 7 species of Scutellaria plants in Taiwan. Additionally, we compared 7 species of Scutellaria plants in Taiwan with the commercial Scutellariae Radix imported from mainland China, evaluating if it is possible to replace the commercial products. The new species, S. hsiehii, contained the 4 main bioactive standard references, which were baicalin, wogonoside, baicalein and wogonin. The average content of 3 samples of baicalin was 4.50±0.17 mg/g, wogonoside was 3.04±0.09 mg/g, baicalein was 0.46±0.02 mg/g, and wogonin was 0.48±0.03 mg/g. According to the analysis of HPLC fingerprint, 7 species of Scutellaria plants in Taiwan have 11 common chromatography peaks, and were generally inclusive the four bioactive components, except S. taiwanensis. On the other hand, the N strong peak number of 7 species of Scutellaria plants in Taiwan were varies from 9 to 11, and the sum of peak area ratio were varies from 67.56% to 81.64%. Among the 7 species, S. tashiroi (81.64%) was the highest one, following by S. austrotaiwanensis (79.87%) and S. hsiehii (78.88%). The commercial Scutellariae Radix and the 7 species of Scutellaria plants in Taiwan have 5 common chromatography peaks, including baicalin, wogonoside and baicalein. In baicalin content analysis, S. hsiehii (4.50±0.17 mg/g), S. tashiroi (4.83±1.00 mg/g) and S. austrotaiwanensis (4.30±0.79 mg/g) were non-inferior the inferior quality commercial Scutellariae Radix (4.07±1.83 mg/g). In wogonoside content analysis, S. hsiehii (3.04±0.09 mg/g) and S. tashiroi (3.16±0.78 mg/g) were equal to the high quality commercial Scutellariae Radix. These results could support further evaluation of S. tashiroi, S. austrotaiwanensis and S. hsiehii as an alternative to S. baicalensis, the commercial Scutellariae Radix imported from mainland China.

參考文獻


1. Harley, R. M., S. Atkins , A. L. Budanstev , 2004 . Flowering plants, dicotyledons. pp. 167-275 in The families and genera of vascular plants Vol. 6 ,ed. K. Kubitzki . Berlin : Springer Verlag .
2. 鄭漢臣, 藥用植物學第三版, 2004, pp 256-258, 台北, 文光圖書有限公司
3. 台灣維管束植物簡誌第四卷, pp 131,147-148, 行政院農業委員會
4. 中華本草, pp 200-210, 上海科學技術出版社
5. 林俊清, 新編本草學第I集, 2004, pp 530-541, 高雄, 富山出版社

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