微流體生醫晶片應用於誘發肺部發炎之趨化蛋白質研究

Microfluidics-based Dynamic Migration Labchip for Studying Protein-induced Lung Inflammation

涂夏爾(Punde Tushar Harishchandra)

指導教授 ： 劉承賢

國立清華大學/工學院/奈米工程與微系統研究所/博士(2015年)

摘要

項研究報告指出一個仿生微系統，其重建成了肺部微環境監測對於肺部炎症嗜酸性白血球陽離子蛋白（ECP）的作用。ECP誘導氣管上皮細胞釋放CXCL-12，反過來又刺激上皮纖維細胞的往氣管上皮細胞遷移。這兩層微流體系統提供了用於灌注培養一個可行的擴散平台，並且被用於本研究顯示，CXCL12-CXCR4為主軸介導的ECP誘導纖維細胞外滲在肺部炎症。
這個'肺上的實驗室“微型裝置可當作是動態的Transwell系統，透過導入流體可以重建血管-組織界面的體外測定，提高臨床前研究用。我們嘗試開發一種新的微流體模型不僅模擬Transwell的細胞遷移，但它也同時可以研究模仿生理條件在體內的遷移。由於血管是我們身體的一部分，這種模式使得研究在涉及血管於器官移植在體外模型更加逼真的機會。

關鍵字

實驗室晶片 ； 肺部發炎 ； 細胞遷移

並列摘要

This study reports a biomimetic microsystem which reconstitutes the lung microenvironment for monitoring the role of eosinophil cationic protein (ECP) in lung inflammation. ECP induces airway epithelial cell expression of CXCL-12, which in turn stimulates the migration of fibrocytes towards the epithelium. This two-layered microfluidic system provides a feasible platform for perfusion culture, and was used in this study to reveal that the CXCL12-CXCR4 axis mediates ECP induced fibrocyte extravasation in lung inflammation.
This ‘lung-on-a-chip’ microdevice serves as a dynamic transwell system by introducing flow that can reconstitute the blood vessel-tissue interface for in vitro assays, enhancing pre-clinical studies. We made an attempt to develop a new microfluidic model which not only simulates the transwell for studying cell migration, but it could also study the migration in the presence of flow mimicking the physiological conditions in the body. As blood vessels are the integral part of our body, this model gives an opportunity to study more realistic in vitro models of organs where blood vessel i.e. flow based migration is involved.

並列關鍵字

Lab on a chip ； lung inflammation ； cell migration

參考文獻

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