明膠與陶瓷複合式支架培養關節軟骨細胞的研究

Study of cultivation of articular chondrocytes on Gelatin and Ceramic composite scaffold

簡千翔(Chien-Hsiang Chien)

指導教授 ： 黃大仁

國立清華大學/工學院/化學工程學系/碩士(2006年)

https://doi.org/10.6843/NTHU.2006.00185

摘要

本研究研發一創新的生醫複合式材料來探討修復關節軟骨的成效，選擇與人體骨組織成份相似的陶瓷材料以及生物相容性良好的明膠天然高分子材料，期望兼具兩者的優點使得此一支架有更佳的培養效果，以及克服一些臨床上的問題。
利用聚磷酸鈣(calcium polyphosphate，CPP)的孔洞來與明膠分子做連結，讓兩者性質大不相同的材質結合起來，表現出來的連結力有相當的水準；輔以β-三鈣磷酸鹽(β-TCP)當作與硬骨細胞培養區的隔離層，避免血管侵入軟骨區影響修復，是一項創新的設計。多重式的支架經過試驗證實陶瓷層不會干擾明膠層關節軟骨細胞的成長，甚至可以抵擋細胞的流失。此外由定性與定量以及RT-PCR實驗發現，體外培養的時間不宜超過二到三週，以免關節軟骨細胞去分化，喪失原有的功能。
醣胺素(Glycosaminoglycan，GAG)中的硫酸鹽軟骨素為關節軟骨組織中重要的成分，其中的一種型態C6S(chondroitin 6 sulfate)，對於其成長有正面的助益。多種與明膠材料的交聯方法中發現，直接加入至培養基中有最大的效應。

關鍵字

生醫陶瓷 ； 明膠 ； 關節軟骨 ； 組織工程 ； 複合式支架

並列摘要

In this study, we developed a novel biomedical composite material to
estimate the effect on the repair of cartilage tissue. We chose the
bioceramic which is similar to the composition of native bone and a
natural polymer-gelatin, which is relatively biocompatible, to make use of
the advantages of each material to improve the cultivation effect and
overcome some clinical problems.
The connected force between calcium polyphosphate(CPP) and
gelatin layer is fairly strong. We used -tricalcium phosphate(-TCP) as
the separation layer to prevent blood vessels from invading the cartilage
zone. After test, it was certified that the growth of chondrocytes on the
gelatin layer were not interrupted by the ceramic material. The qualitative,
quantitative and RT-PCR data showed that the period of in-vitro culture
should not be over two or three weeks; otherwise, the differentiation of
chondrocytes will occur.
Chondroitin sulfate is an important component of cartilage tissue; it
profits the growth of the tissue. We figured out that pouring C6S into the
medium was the best among methods of cross-linking with gelatin.

參考文獻

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