柴胡BkERF1基因表現分析及其蛋白質之表現

Transcription analysis and recombinant protein expression for the Bupleurum kaoi encoding BkERF1 gene

廖珮妤(Pei-Yu Liao)

指導教授 ： 林彩雲

國立清華大學/生命科學院/生物資訊與結構生物研究所/碩士(2007年)

摘要

本研究分析了實驗室先前所分離的高氏柴胡BkERF1基因之轉錄並表現其重組蛋白質，依據ERF保留區段的結構，BkERF1蛋白質可歸類為ERF蛋白質的subcluster B3及class III之成員。預測BkERF1內的保守度高的Arg83, 85, 95, 103與Trp87, 105 是GCC box的結合位置。BkERF1 在高氏柴胡的不同器官皆可表現。雖然MeJA或ACC處理只稍微增加高氏柴胡組培根 BkERF1的轉錄，然而這兩者荷爾蒙的組合導致顯著的協同效應使BkERF1轉錄大幅增加，顯示BkERF1對於MeJA及ET交互訊息傳導途徑的重要性。

關鍵字

高氏柴胡 ； 乙烯回應因子

並列摘要

Transcription of the previously isolated Bupleurum kaoi gene, BkERF1, was analyzed and the recombinant protein was expressed in this study. The BkERF1 protein which contains conserved ethylene responsive transcriptional factor domain is categorized to the member of subcluster B3 and class III of the ERF proteins based on structural organization. The conserved residues Arg83, 85, 95, 103 and Trp87, 105 in BkERF1 are predicted to be the GCC box binding site. BkERF1 was found to constitutively express in all different organs of B. kaoi. Though MeJA or ACC alone slightly increased the transcription of BkERF1 in B. kaoi adventitious roots, a combination of these two hormones resulted in a synergistic effect on the increase of BkERF1 transcripts. These results suggest that the importance of BkERF1 in the regulation of MeJA and ET cross-talk signaling pathway.

並列關鍵字

Bupleurum kaoi ； ethylene-responsive factor

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