多功能隨機存取式微液滴反應器陣列晶片之開發

A Random-Access Microarray for Programmable Droplet Storing, Retrieval and Manipulation

曾逸銘

指導教授 ： 蘇育全 ； 曾繁根

國立清華大學/原子科學院/工程與系統科學系/碩士(2011年)

https://doi.org/10.6843/NTHU.2011.00145

摘要

數位微流體系統為在近年來越來越受注目的研究領域之一，由於它擁有快速、精準、大量且自動化的優點，使得在化學、生物及醫學領域的應用上有著極大的潛力。利用數位微流體系統的特性，可把不同反應的實驗，置入在各自獨立且微小的微液珠中進行，同時在微小化與平行化的系統晶片中，做出各種實驗所需功能動作，在完成各種生化反應實驗後的微液珠可視為一組實驗樣本，再透過本研究所提供的定址存取系統晶片平台，加以儲存，透過此晶片平台進行長期觀察、篩選，甚至在日後樣品取樣，皆為本實驗研究的目標成果。希望透過此多功能隨機存取式晶片所具有微量檢測、精準定量、快速平行處理及自動化操控的優點，加快和便利在各種生物、化學和醫學研究實驗上的突破與發展。在本研究論文提出一種可依實驗需求而達到任意存入取出微液珠的多功能陣列式晶片，此晶片為多層PDMS微結構元件，利用氣動式薄膜來控制兩層微流道結構，在數位微流道控制的概念下，透過特殊設計的微流道暫存單元(microwell)陣列，來達到指定微液珠定址在微流道陣列晶片中的特定暫存單元，並達成微液珠在微流道晶片中展現任意定址存入取出的功能。在實驗原型晶片中，可依實驗需求製作所需要成份與定量的微液珠，並選擇性地存入4×4的目標暫存單元內或從中取出，並且現階段可達到微量合成、長期觀察、選擇指定樣品目標的功能，進階的在原型晶片陣列(4×4)只須要4 個(=2×log24)控制訊號即可。隨著此控制訊號與晶片設計發展，能實現更大數量級的陣列晶片，未來可應用在生化分析領域上，尤其可應用在快速合成藥物與成份檢體篩檢(screening)的領域上。

關鍵字

數位微流體 ； 微陣列 ； 微液珠 ； 儲存 ； 篩檢

並列摘要

This thesis presented a multi-function microfluidic array that is capable of programmably metering, entrapping, coalescing, addressably storing and retrieving droplets, which could potentially serve as reactors for continuous tracking and multi-step processing. A PDMS multi-layer chip with specially designed fluidic-channels dynamically re-configured by pneumatically-actuated diaphragms is utilized to realize various droplet manipulation schemes. We have fabricated the prototype demonstration that droplets with desired volumes and compositions are generated. Once droplets are formed, their motions are coordinated by a 2-D multiplexing scheme, which exploits the bi-directional movement of diaphragms to implement a random-access microarray, which means our microarray chip could be selectively stored, and retrieved from a 4×4 array, which employs just 4 (=2×log24) control inputs for the operation. With droplets functioning as micro-reactors, have the advantages such as low sample consumption and high reaction that the proposed random-access storage array could potentially serve as a chip for high throughput and multi-step reaction control. The chip is expected to significantly accelerate the progress in drug discovery, and various chemical and biological screening and synthesis. There are three accomplishments have been achieved in this thesis paper : (1) 2-D multiplexing can be largely facilitated using bi-directional diaphragm valves, (2) N×N multiplexing of droplets could be realized utilizing only 2×log2N control inputs and (3) a fully addressable, random-access array can be accomplished employing multi-layer microarray PDMS chip.

並列關鍵字

Random-Access Microarray ； 2-D Multiplexing ； Microfluidic ； Droplet ； Storing ； Retrieval ； Screening

參考文獻

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