果蠅睡眠監控平台與濕度和氣味干擾實驗

Drosophila Sleep Monitor Platform and Disturbance Experiment of Humidity and Odor

宋宜芸(Sung, Yi Yun)

指導教授 ： 賀陳弘

國立清華大學/工學院/動力機械工程學系/碩士(2012年)

摘要

近年來在果蠅腦科學研究上，多以人工的方式來進行實驗，本論文改良舊有多重刺激源睡眠監控平台，輔以自動化程式控制，將刺激源模組化，並使用氣味及濕度做為主要刺激源，用非接觸的方式降低果蠅可能因外力而造成的傷害，在夜晚給予刺激源干擾來剝奪果蠅的睡眠。在實驗分析方面，則用紅外線攝影機錄下果蠅晝夜活動情形，配合影像分析做後處理，來觀察受刺激源干擾後的睡眠情形。實驗使用四種果蠅喜惡性較明顯之氣味，分別為香蕉油、蘋果醋、OCT、BA；利用常溫下50%~60%以及80%~90%相對濕度，來探討環境中的濕度對於果蠅睡眠行為的影響。未來期望此創新的多重刺激源睡眠監控平台，能在果蠅睡眠研究方面，提供更多的行為研究模型，同時可藉由多重刺激源模組功能，使日後科學家在果蠅神經及基因方面，做更加深入的了解。

關鍵字

果蠅 ； 睡眠 ； 氣味 ； 濕度

並列摘要

In recent years, neurogenetics research of drosophila experiment is more needed. In this thesis, novel sleep monitoring platform which is capable of installing multiple stimulation modules and automatic monitoring of sleep is developed. The stimulation modules are controlled by LabVIEW programs.
This study gave the odorant and humidity stimuli to disturb the sleep of flies in the night. The physical damage is minimal using the non-contact method. The sleep behavior video was recorded by Infrared camera to provide drosophila sleep data.
The novel sleep monitoring platform provides a new neurogenetics research tool in drosophila sleep. In the future, scientists are able to develop effective medicine to help people who suffers from insomnia.

並列關鍵字

Drosophila ； odorant ； humidity ； sleep

參考文獻

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