大鼠大腦皮質區內具有鈣離子通透型AMPA受器的神經細胞以及能夠與丘腦產生聯繫的神經細胞在發育時期經過的改變

Developmental changes in calcium-permeable AMPA receptor-expressing neurons and corticothalamic neurons in the rat neocortex

許正宜(Cheng-I Hsu)

指導教授 ： 張兗君

國立清華大學/生命科學院/分子醫學研究所/博士(2009年)

https://doi.org/10.6843/NTHU.2009.00772

摘要

ABSTRACT
The neocortex is a highly specialized region in adult mammalian brain. A largely transient population of neurons localizing in the subplate has been proposed to play crucial roles in cortical development. In the first part of this thesis, I aimed to study the distribution of calcium permeable AMPA receptor (CP-AMPAR)-expressing neurons in subplate and neocortex during development and the roles played by CP-AMPARs in the death of subplate neurons. The kainate-stimulated Co2+-labeling assay was used to identify the CP-AMPAR-expressing neurons. The pretreatment of kainate coupled with the Co2+-labeling assay was used to detect the susceptibility to excitotoxicity of CP-AMPAR-expressing neurons. The widespread distribution and distinct susceptibility to excitotoxicity of CP-AMPAR-expressing neurons suggest that CP-AMPAR-expressing neurons play various important roles in the development and physiology of the rat cerebral cortex.
The neocortex and thalamus develop synchronously and link with each other through reciprocal connections, the thalamocortical and corticothalamic (CT) projections. In the second part of this thesis, the morphology of DiI-back-labeled CT neurons in perinatal rat cortices was studied. The immunoreactive signals of reelin, calbindin, GluR1, gephyrin and synaptophysin at the apical dendrites of CT neurons in perinatal rat cortices were analyzed. The results suggest that the apical dendrites of the CT neurons can form contacts with Cajal-Retzius (CR) and other cells in the marginal zone or upper cortical plate at E18. Furthermore, GABAergic and glutamatergic synapses are already formed on CT neurons in rat cortices as early as E18 when the cortical projection has just reached their thalamic targets. Thus, it is likely that the cortex and thalamus in rat brains are linked to each other by functional connections in a two-way fashion at least three days before birth.

關鍵字

麩胺酸受器 ； 鈣離子 ； 大腦皮質 ； 發育

並列摘要

無資料

並列關鍵字

AMPA receptor ； calcium permeability ； cobalt labeling ； corticothalamic neurons ； somatosensorycortex ； development

參考文獻

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Ben-Ari Y. 2001. Developing networks play a similar melody. Trends Neurosci 24(6):353-360.

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Higashi S, Molnar Z, Kurotani T, Toyama K. 2002. Prenatal development of neural excitation in rat thalamocortical projections studied by optical recording. Neuroscience 115(4):1231-1246.