π－π 作用力之毛細管電層析法分離多環芳香烴化合物

Separation of Polycyclic Aromatic Hydrocarbons Based on π-π Interaction by Capillary Electrochromatography

黃星皓(Sing-Hao Huang)

指導教授 ： 黃悉雅

中原大學/理學院/化學研究所/碩士(2012年)

https://doi.org/10.6840/cycu201200578

摘要

本篇論文進行毛細管電層析技術的研究，本實驗是以烷基甲基丙烯酸
酯(alkyl methacrylate)單體，結合二乙烯苯(divinylbenzene, DVB)作為交聯劑
聚合而成的整體成形材料，作為毛細管電層析(capillary
electrochromatography, CEC)管柱之靜相研究。研究探討六種不同苯環數目
之多環芳香族化合物(polycyclic aromatic hydrocarbons, PAHs)與靜相之間的
作用關係。測試的PAHs 分析物，具有豐富的π 電子，能與靜相中的DVB
呈現強烈的π－π 作用力，然而過於強烈的作用力會造成滯留時間過長及波
峰拖尾，而波峰拖尾會造成靈敏度下降、精確度差、定量困難以及解析度
差等缺點。研究中藉由改變烷基甲基丙烯酸酯單體的鏈長以及與DVB 交聯
劑的含量，探討對於分析物滯留時間、滯留因素以及拖尾因子的影響。接
著將整體成形管柱，應用於國際法規(美、德)規範具致癌性的16 個PAHs
化合物上，並結合大氣壓力化學離子源(atmospheric pressure chemical
ionization, APCI)串聯至離子阱質譜儀系統，使這16 個PAHs 的偵測極限
(limit of detection)能降低至0.0011-0.0375ppm 以達到法規所規範範圍。

關鍵字

大氣壓力化學離子源 ； 毛細管電層析

並列摘要

In the thesis, capillary electrochromatography (CEC) technique was
studied. Poly(alkyll methacrylate-co-divinylbenzene) based monolith as the
stationary phase were studied, which were used to investigate the relationship
between the stationary phases and the six different aromatic rings of polycyclic
aromatic hydrocarbons (PAHs). PAHs have a wealth of π electrons, showing the
strong π-π interaction with DVB. However, this interaction resulted to long
retention time and peak tailing which eventually resulted to decreased sensitivity,
poor precision, difficulty in quantitation and poor resolution. Therefore, the type
and the ratio of alkyl methacrylates with respect to the DVB crosslinker were
optimized and the effect of these parameters on the retention time, retention
factor and tailing factor was studied. Moreover, the optimized parameters were
applied to the 16 types of PAHs using atmospheric pressure chemical ionization
(APCI) tandem ion trap mass spectrometer system. The detection limit of the 16
PAHs can be reduced to 0.0011-0.0375 ppm which are within the specification
range set by the regulatory agencies of Germany and the United States (0.1ppm)
for these carcinogens.

並列關鍵字

atmospheric pressure chemical ionization ； capillary electrochromatography

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