紅光對不朽化人類骨髓間葉幹細胞增生及硬骨分化之影響

The Effects of Red-light Irradiation on the Proliferation and Osteogenesis of Immortalized Mesenchymal Stem Cells Derived from Human Bone Marrow

程士瑋(Shi-Wei Cheng)

指導教授 ： 李文婷

中原大學/工學院/醫學工程研究所/碩士(2007年)

https://doi.org/10.6840/CYCU.2007.00656

摘要

低能量光照具有促進細胞增生、加速傷口癒合及調控細胞活性的特性，目前各界正積極探討其對於各種生物細胞之影響。人類骨髓間葉幹細胞 ( human bone marrow mesenchymal stem cells，hBMSC ) 因具有多向性分化能力，具有醫療潛能，但在臨床應用上常有數目不足的問題，如何促進 MSC 之增生，為目前幹細胞研究的重點之一。本研究將探討紅光 ( 660 nm ) 發光二極體 ( Light emitting diode，LED ) 光照刺激對於 hBMSC 增生及硬骨分化的影響。實驗結果發現以5 mW/cm2、1 J/cm2單次光照，及 10 mW/cm2、4 J/cm2 連續光照刺激，可促進以 2 % FBS培養之 hBMSC 之生長速率；而 10 mW/cm2、2 J/cm2連續光照刺激，可促進以10 % FBS培養之 hBMSC之生長。而在光照刺激 hBMSC 形成CFU-F個數之結果發現，光照刺激參數：5 及10 mW/cm2 ，1、2及4 J/cm2 連續光照刺激和 10 mW/cm2， 1 J/cm2單次光照刺激，均可促進 hBMSC 形成 CFU-F個數的增加，其中以 10 mW/cm2 ，1、2及4 J/cm2 連續光照此三種參數表現最佳。利用此三種參數刺激 hBMSC 硬骨分化，發現能量1 J/cm2 連續光照，可以使 hBMSC 之鹼性磷酸酶活性提高，此研究結果證明某些光照參數可以增加 hBMSC 形成 CFU-F 個數和促進硬骨分化

關鍵字

間葉幹細胞 ； 發光二極體 ； 硬骨分化 ； 低能量光照

並列摘要

Low level light irradiation is known to increase cell growth, promote wound healing, and modulate cellular activities. The effects of photostimulation on cells are under fervent study. Human bone marrow mesenchymal stem cells (hBMSC) have the characteristics of multi-lineage of differentiation, which can be applied in clinical application. One of the major difficulties is to promote cell growth. It is of great importance to explore various approaches to obtain meaningful number of cells for medical clinical treatments. The purpose of the study was to investigate the effects of red-light (660 nm) light emitting diode (LED) irradiation on the growth and osteogenesis of hBMSC. The results found that single exposure of irradiation (5 mW/cm2, 1 J/cm2) and multiple exposures of irradiation (10 mW/cm2, 4 J/cm2) could result in the increase of growth of cells incubated in the medium with 2 % FBS. Daily irradiation (10 mW/cm2, 2 J/cm2) could increase the growth of hBMSC incubated in the medium with 10 % FBS. According to the results of CFU-F assay, the increase of CFU-F number was observed after light irradiation at the parameters of 5 and 10 mW/cm2, and 1, 2 and 4 J/cm2 (continuous exposure) as well as 10 mW/cm2, and 1 J/cm2 (single exposure). The maximum CFU-F number increase was at irradiance of 10 mW/cm2, and radiant exposure of 1, 2 and 4 J/cm2 (continuous exposure). Alkaline phosphates activity of hBMSC was found to increase when treated with continuous exposure of 1 J/cm2 irradiation. Our results demonstrate that the colony forming potential and osteogenic differentiation of hBMSC could be enhanced by 660 nm LED irradiation under specific parameters.

並列關鍵字

low level light irradiation ； mesenchymal stem cell ； light

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