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  • 學位論文

陰電性聚氟化乙二烯薄膜之表面親和性控制及其於特定蛋白質辨識與分離效能之研究

Surface affinity control of PEGylated poly(vinylidene fluoride) membrane grafted with cationic 3-Sulfopropyl methacrylate for specific protein recognition and separation

指導教授 : ARRAY(0xa9fd798)

摘要


在本研究中,將以聚二氟乙烯(polyvinylidene fluoride, PVDF)薄膜作為基材,使用3-磺酸丙基甲基丙烯酸甲鹽(3-Sulfopropyl methacrylate potassium salt, SA)與甲基丙烯酸聚乙二醇酯(poly ethylene glycol methacrylate, PEGMA)兩種單體經由下述三個步驟將其接枝於薄膜表面上來製作出具有陽離子交換能力的吸附薄膜:1. 將PVDF薄膜浸泡於含有不同單體莫耳比例的溶液中進行物理吸附;2. 將薄膜風乾後,以低壓氬氣電漿在不同處理時間下利用電漿接枝法使塗佈於薄膜表面上的單體與薄膜之間進行接枝反應;3. 利用超聲波清洗將因自聚或未接枝於薄膜上的單體或聚合物洗出。 吸附實驗是以溶菌酶及牛血清蛋白作為測試蛋白,以不同單體莫耳比例、電漿處理時間、溶液pH值以及離子強度作為靜態吸附實驗的操作參數來觀察改質薄膜的吸附能力。在動態吸附實驗以不同流速觀察其對蛋白質吸附能力的影響。 研究結果顯示,在電漿處理時間為90秒,SA莫耳比例為30 %以及單體重量百分比為30 wt%時,改質薄膜有較高的靜態目標蛋白吸附量以及LY ratio,而在單成份蛋白質動態吸附實驗方面,流速改變不會影響吸附材對單成分蛋白質的吸附及分離效果,且單體重量百分比為5 wt%時較30 wt%的改質薄膜擁有較高的純水通透量及相近的蛋白質吸附量,而在雙成分蛋白質動態吸附實驗中發現,改質薄膜可成功將溶菌酶與牛血清蛋白分離開來。

並列摘要


In this study, poly (vinylidene fluoride) microfiltration membrane was used as based material. Two monomers (3-Sulfopropyl methacrylate potassium salt, SA and poly (ethylene glycol) methacrylate, PEGMA) were grafting on PVDF by three steps to create a cation-exchange adsorption membrane: (i) immersing PVDF membranes in the different molar ratio monomer solutions as physical coating. (ii) dried membranes were modified with low pressure argon plasma with various time.(iii) using ultrasonic to wash out non-grafting monomers on the membranes. Lysozyme and BSA used as model proteins to test the adsorption ability, moreover, different monomer molar ratio, plasma treated time, monomer weight percentage, pH value and ion strength and were used for parameters of static adsorption experiment. In dynamic adsorption experiment, different flow rate was used to check the effect on the protein capacity. The result shows that, when plasma treated time was 90 sec and the SA molar ratio was 30 %, the modified membrane has higher target protein static adsorption capacity and LY ratio at 30 wt% monomer weight percentage. In single protein dynamic adsorption experiment, the capacity and separation efficiency were independent with flow rate. From binary dynamic protein adsorption experiment result, we find the modified membrane can successfully separate LY and BSA.

參考文獻


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被引用紀錄


劉沛謙(2015)。常壓電漿接枝與紫外光接枝雙離子性材料於高分子薄膜改質之比較研究〔碩士論文,中原大學〕。華藝線上圖書館。https://doi.org/10.6840%2fcycu201500666
施曉琳(2014)。常壓電漿接枝類雙離子材料於高分子薄膜之研究〔碩士論文,中原大學〕。華藝線上圖書館。https://doi.org/10.6840%2fcycu201400497
許馨勻(2012)。常壓電漿製備酸鹼敏感與抗生物沾黏薄膜之研究〔碩士論文,中原大學〕。華藝線上圖書館。https://doi.org/10.6840%2fcycu201200648
蔡怡妮(2011)。平板式常壓電漿快速接枝甲基丙烯酸聚乙二醇 酯在聚偏二氟乙烯薄膜之研究〔碩士論文,中原大學〕。華藝線上圖書館。https://doi.org/10.6840%2fcycu201100654

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