製備磺酸化梳狀聚甲基丙基酸環氧酯與蛋白質吸附之應用

Preparation and application of PGMA brushes carry taurine for protein adsorption

徐培鈞(Pei-Chun Hsu)

指導教授 ： 許克瀛

中原大學/工學院/化學工程研究所/碩士(2010年)

https://doi.org/10.6840/cycu201000799

摘要

本研究利用無乳化劑乳化聚合反應製備聚氯甲基苯乙烯顆粒，做為大分子起始劑。再利用原子轉移自由基聚合法將甲基丙基酸環氧酯接枝在顆粒表面上，形成梳子狀高分子顆粒，並將接枝後顆粒磺酸化，應用在蛋白質吸附。利用單體固體含量及反應條件的改變，探討對聚氯甲基苯乙烯粒徑大小，PVBC-g-GMA顆粒的表面形態、粒徑大小及表面電位，磺酸化顆粒對蛋白質的吸附量及再使用性測試。
由實驗結果得知，聚氯甲苯乙烯顆粒粒徑隨親水性共單體增加而減小。在ATRP反應溶劑中加入少量的水，可使聚合反應速率增加，在一定反應時間內GMA接枝量隨之增加。接枝後的顆粒表面電位會隨著GMA接枝量增加而減少。蛋白質吸附測試中，吸附量隨著接枝量增加而增加，在pH值為11時，蛋白質具有最大吸附量，吸附量大約為432mg/g，且顆粒經過六次重複使用測試，吸附效率還可高達90%。

關鍵字

蛋白質吸附 ； 無乳化劑乳化聚合法 ； 原子轉化自由基聚合法

並列摘要

In this study, poly(vinylbenzyl chloride)(PVBC) particles were prepared by emulsifier-free emulsion polymerization, as macromolecular initiator. There particle were grafted from with poly(glycidyl methacrylate) via atom transfer radical polymerization (ATRP). Epoxy groups were replace by taurine (NH2CH2CH2SO3H) to. There particle surface were possessed sulfonic groups.
By changing the recipes and reaction conditions, the effects on the PVBC particle size and PVBC-g-GMA particle morphology were investigated. There sulfonated particles were used for protein adsorption. The possibility of re-use was considerd and proceeded.
From the experimental results, hydrophilic comonomer(NaSS) increases with PVBC particle size, add small amount of water in the ATRP reaction, polymerization rate can be accelerated and the amount of GMA grafting on particles also increased. The surface potential energy of decreased as the increase of GMA grafting.
The protein adsorption increased as the increase of GMA grafting. The maximum adsorption is around 432mg/g. After six re-use test, the adsorption efficiency was still up to 90%.

並列關鍵字

Protein adsorption ； ATRP ； Emulsifier-free emulsion polymerization

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