研究雙離子型薄膜的快速表面生物惰性功能化及探討用於血球細胞選擇的智能生物活性界面

Rapid surface bioinert functionalization of zwitterionic membranes and intelligent bioactive interfaces for blood cell selection

林秀芳(Hsiu-Fang Lin)

指導教授 ： 張雍

中原大學/工學院/化學工程研究所/碩士(2021年)

https://doi.org/10.6840/cycu202100464

摘要

本論文第一部份的研究中，我們成功將磺基甜菜鹼甲基丙烯醯胺(sulfobetaine methacrylamide, SBAA)和甲基丙烯酸丁酯(butyl methacrylate,BMA)所聚合製備之poly(SBAA-co-BMA)雙離子共聚物透過超音波微粒表面噴塗工藝， 改質於已經過電漿預處理的拉伸聚四氟乙烯(epanded Polytetrafluoroethylenee, ePTFE)薄膜，進而獲得應用於糖尿病傷口的敷料。利用表面接觸角量測儀、介面電位分析儀、掃描電子顯微鏡和全反射式傅里葉紅外線光譜儀等方式鑑定與分析薄膜的親水性、表面電位、物理結構與化學組成。發現未經電漿預處理的ePTFE 薄膜，其poly(SBAA-co-BMA)高分子塗層容易因為應力不均而有裂開甚至剝離的現象。在動物實驗和組織切片分析結果表明，經塗覆參數優化後的雙離子ePTFE 薄膜可用於一型糖尿病傷口復原的控制。傷口癒合的速度和新生成的組織表現不僅比未改質的薄膜更好，並且其恢復率還優於已商業化敷料。因此，透過電漿預處理先將
ePTFE 薄膜提高親水性，並再使用超音波微粒噴塗技術將poly(SBAA-co-BMA)改質於ePTFE 薄膜上，能有效製備出促進慢性傷口癒合的新式敷料，為化學惰性材料改質方法提供新製備途徑與具有潛力的醫療應用。
本論文第二部份的研究中，我們成功製備兼具溫感響應及專一性篩選白血球的水膠材料。在合成水膠後，以全反射傅里葉紅外線光譜儀進行化學組成分析。並使用雷射共軛聚焦顯微鏡觀察白血球於水膠吸脫附前後的差異。本研究發現含有羧基的丙烯酸(Acrylic acid, AAc)能有效貼附白血球，並藉由混摻適量的溫感性材料N-異丙基丙烯醯胺(N-isopropylacrylamide,NIPAAm)可提供有效脫附白血球的功能。因此，由AAc 及NIPAAm 製備的智能型水膠有潛力成為可吸脫附白血球細胞的新型生物活性界面材料系統。

關鍵字

聚四氟乙烯 ； 超音波微粒噴塗 ； 雙離子 ； 糖尿病傷口 ； 水膠 ； 溫度敏感性 ； 白血球脫附

並列摘要

In the first part of the research, we successfully modified the plasmapretreated
ePTFE membranes by means of utilizing butyl methacrylate and sulfobetaine methacrylamide (PBMA-co-PSBAA) antifouling copolymer through the ultrasonic surface coating process to obtain a dressing for diabetic wounds.The surface wettability, zeta potential and chemical composition of the membrane were measured by contact angle meter (CA),zeta potential analyzer, reflection Fourier Transform Infraed Spectromete (FTIR),and scanning electron microscope(SEM), respectively. The coating of ePTFE membrane without plasma pretreatment was observed to be fragile and non-homogeneous which led to crack and spallation. The results of animal experiments and histological analysis indicated that the optimized zwitterionic ePTFE membrane can be exploited to control the type Ⅰ diabetic wounds. The wound healing speed and newly formed tissues demonstrated not only exceptional performance but also potential properties of commercial wound dressings. Therefore, through plasma pretreatment, the hydrophilicity of the ePTFE membrane is improved, and then the poly(SBAA-co-BMA) is modified on the ePTFE membrane by ultrasonic particle spraying technology, which can effectively prepare a new dressing that promotes the healing of chronic wounds.
In the second part of the study, we successfully prepared the thermosensitive hydrogels which is specific for blood cell selection .The chemical compositions of hydrogel were examined by FTIR. Confocal laser scanning microscopy (CLSM) was selected to observe the attachment of WBCs on the hydrogel. To conclude, it was found that the carboxyl groups of Acrylic acid (AAc) can effectively capture WBCs, in addition, appropriate amount of temperature sensitive material N-isopropylacrylamide (NIPAAm) can effectively detach WBCs. Therefore, the smart hydrogel prepared by AAc and NIPAAm has the potential to become a new type of bioactive interface material system that can attach and detach WBCs.

並列關鍵字

ePTFE ； ultrasonic spray ； zwitterionic ； diabetic wound ； hydrogel ； thermo-sensitive ； leukocyte detachment

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