應用辛烯基丁二酸兩性分子修飾磷化銦/硫化鋅量子點生醫界面化學之探討

於奈米科技領域中，由於量子點擁有獨特之光學特性，於近幾年間，逐漸取代傳統之有機螢光染劑、綠螢光蛋白，亦於相關生醫領域趨於成熟，如細胞標定、細胞追蹤。但合成傳統量子點之材料為重金屬(鎘離子、鉛離子)，其對於生物及環境皆會帶來嚴重毒性汙染。科學家紛紛提出不同材料取代重金屬離子，進而提高其生醫之應用。本研究主要為合成有機疏水相之磷化銦/硫化鋅無鎘量子點，並利用辛烯基丁二酸製備成相轉換之微乳化劑，主要用以取代傳統具有生物毒性之界面活性劑。利用簡單、快速之微乳化相轉換方式，不只可將磷化銦/硫化鋅無鎘量子點成功轉換成親水相結構，亦可將其它有機疏水之奈米粒子轉換成親水相之結構。但由辛烯基丁二酸所提供之相轉換緩衝層結構不穩定，容易產生崩解，本實驗發現先與甲氧基聚乙二醇反應後，提高其結構之穩定性。隨後，以二硬脂酰基磷脂酰乙醇胺-聚乙二醇-胺基(-NH2)、羧基(-COOH)、甲氧基(-Methoxy)進行表面接枝，並以水平電泳驗證其表面接枝。另外，以二硬脂酰基磷脂酰乙醇胺-聚乙二醇-生物素(-Biotin)進行表面接枝，與具有卵白素(Streptavidin)基板反應後，以原子力顯微鏡進行表面接枝之驗證。故本研究提出可利用生物相容性極佳之辛烯基丁二酸微乳化劑，對有機相磷化銦/硫化鋅無鎘量子點進行親水相之改質，並利用甲氧基聚乙二醇增加緩衝層之穩定性，改善相轉換後伴隨之結構不穩定狀況，提升後續生醫應用之價值。

關鍵字

辛烯基丁二酸；磷化銦/硫化鋅；微乳化

並列摘要

Recently, the unique optical properties of quantum dot gradually replace the traditional organic fluorescent dyes and fluorescent proteins in the field of biomedical technology. It has become important tools in related biomedical areas, such as cell targeting, cell tracking. But the material for the synthesis of traditional quantum dot contains heavy metal, such as Cd2+、Pb2+, which will cause serious toxicity for both biological and environmental pollution. Scientists have investigated the alternative materials to replace heavy metal ions and thereby increase its potential in biomedical applications. This study focused on engineering hydrophobic InP/ZnS quantum dots and on the use of octenyl succinic anhydride (OSA) toward bioconjugation. Using a simple, fast microemulsion method not only can modify the hydrophobic InP/ZnS Quantum dots to be hydrophilic ones but also transfer versatile hydrophobic nanoparticles to aqueous phase successfully. However, the buffer layer structure conposed by the octenyl succinic anhydride was unsteady, it was prone to result in disintegration in diluted condition. Then we further showed that using polyethylene glycol monomethyl ether as the surface modifier can increase the stability of InP/ZnS hydrophilic structure. Subsequently, using DSPE-PEG-X (X: -amine, -carboxyl, -methoxy) can further functionalized the surface which can be verified by gel electrophoresis. In this study, we found that the use of octenyl succinic anhydride can offer superior hydrophilic modification of InP/ZnS quantum dots and that the use of polyethylene glycol monomethyl ether can increase stability of buffer layer to improve the stability after phase transfer. We envision the coating method will expand the nanomaterials applying in biomecial research.

並列關鍵字

InP/ZnS quantum dots ； octenyl succinic anhydride ； microemulsion

參考文獻

39 Wang, J. D., Han, S. M., Ke, D. D. & Wang, R. B. Semiconductor Quantum Dots Surface Modification for Potential Cancer Diagnostic and Therapeutic Applications. Journal of Nanomaterials,1-8,(2012).

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國際替代計量

應用辛烯基丁二酸兩性分子修飾磷化銦/硫化鋅量子點生醫界面化學之探討

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