螢光金奈米團簇於神經幹細胞之應用

金奈米粒子具有良好的生物相容性，被廣泛應用於醫學上的檢測、疾病診斷、基因偵測。另外，金奈米粒子也是人體內可存在的一種微量細胞調節輔酶，能促進多種細胞生長分化、促進血管及內皮細胞增殖，在進入細胞後，能調節細胞功能。近年來有研究報導指出，帶負電的金奈米粒子可導正阿茲海默症關鍵蛋白的錯誤摺疊。馬偕醫院的葉宏一教授發現螢光金奈米團簇可降低血管內皮細胞的氧化壓力，本篇研究主要探討螢光金奈米團簇（Fluorescent gold nanoclusters, FANCs）對於體外培養神經幹細胞（Neural stem cells，NSCs）及PC-12細胞其抗氧化壓力、細胞增生及分化的影響。神經幹細胞具有自我更新、分化的潛能，在神經系統疾病或神經的損傷修復，神經幹細胞扮演重要角色。PC-12細胞具有被神經生長因子誘導分化為類神經細胞的特性，長期被廣泛應用作為神經科學相關研究的體外模型。本研究利用神經幹細胞、PC-12細胞與接枝硫辛酸的FANCs共同培養，觀察FANCs與NSCs、PC-12細胞培養後，不同濃度的FANCs對NSCs、PC-12細胞型態之影響、進行FANCs對細胞增生分析及偵測活性氧族群變化和機轉。結果顯示，在200 nM濃度下可有效幫助細胞降低氧化壓力，並促進細胞之增生，然而在400 nM以上會對細胞的增生情況造成影響。接著，利用濃度200 nM對細胞進行分化實驗，結果顯示200 nM的FANCs會延緩細胞分化能力。

關鍵字

神經幹細胞； PC-12細胞；螢光金奈米團簇；分化；增生

並列摘要

Gold nanoparticles are widely used in the diagnosis of tumors, and gene detection owing to their good biocompatibility. In addition, gold nanoparticles may be as a tracer cell regulation coenzyme present in the human body, can promote a variety of cell growth, differentiation and vascular endothelial cell proliferation. After it entering the cell, can regulate cell functions, stimulates fibroblasts and secreting the epidermal growth factor. In the recent years, neurotoxic studies have reported that negatively charged gold nanoparticles may lead to misfolded proteins which are the key to Alzheimer disease. This study investigated the effects of fluorescent gold nanoclusters (FANCs) for its anti-oxidative properties, proliferation and differentiation in neural stem cells (NSCs) and PC-12 cells. NSCs self-renewal and differentiation potential which playing an important role on neurological diseases or nerve damage repair. PC-12 cells were induced by nerve growth factor and differentiated into characteristics of neural cells, is widely used as an in vitro model of neuroscience research. Herein, we present the NSCs and PC-12 cell lines were cultured with various concentrations of FANCs which are grafted with lipoic acid (DHLA, dihydrolipoic acid). Cell morphology and cell proliferations were observed using fluorescent microscopy and detection of reactive oxygen species mechanisms by flow cytometry. The results showed that the concentration up to 400 nM will caused the damaged to the NSC and PC-12 cells. On the other hand, 200 nM FANCs promotes the cell proliferation and inhibites the cell differentiation.

並列關鍵字

Fluorescent gold nanoclusters ； Neural stem cells ； differetiation ； proliferation ； PC-12 cell

參考文獻

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螢光金奈米團簇於神經幹細胞之應用

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