Chlorine can be used to sterilize drinking water. But drinking water contain chlorine compound may be harmful to our human body. According to the survey reported by US Environmental Protection Agency, drinking water after chlorination for sterilization will contain chloroform, with the average concentration of 20μg/L and the maximum concentration of about 311μg/L. Therefore, this study proposed the use of spectrometer to determine whether drink water containing chloroform. Results showed that spectrometric analysis can determine the concentration of chloroform at drinking water.