規律運動配合硫辛酸增補對體內超氧離子歧化酶活性的影響

The Influence of Regular Exercise and Alpha Lipoic Acid Supplementation on the Superoxide Dismutase Activity in Human

吳曉暢(Hsiao-Chang Wu)

指導教授 ： 謝伸裕

國立臺灣師範大學/運動與休閒學院/體育學系/碩士(2007年)

摘要

目的：探討規律運動、抗氧化劑硫辛酸增補對體內超氧離子歧化?活性的影響。方法：受試者共分為四組：服用安慰劑無規律運動習慣組（PN組）、服用安慰劑有規律運動習慣組（PE組）、增補硫辛酸無規律運動習慣增補組（AN組）及增補硫辛酸有規律運動習慣組（AE組），每組10位男性受試者，共計40人。研究中共進行2次衰竭性運動測試（Bruce potocol），兩次運動測試間隔2周予以增補硫辛酸或安慰劑之實驗處理，每次運動測試前、衰竭時、運動後15分鐘、運動後30分鐘分別採集血液樣本，分析血球超氧離子歧化?活性。所得數據以混合設計雙因子變異數分析進行檢定。結果：第一次運動測試中，在衰竭及運動後15分鐘時，有規律運動習慣者（PE、AE組）血球SOD活性顯著高於無規律運動習慣者（PN、AN組）；第二次運動測試中，衰竭時PE、AE組皆顯著高於PN、AN組；兩次運動測試間運動表現無顯著差異，SOD活性表現差異未達顯著；AE組與AN組第一次運動測試衰竭時無顯著差異（p = .079），第二次運動測試時則達顯著差異(p = .002)，且AE組SOD活性峰值由運動後15分鐘前移至衰竭時，衰竭時SOD活性第二次運動測試顯著高於第一次（p = .035）。結論：連續兩週的抗氧化劑硫辛酸增補，對於有規律運動習慣者，雖無顯著SOD活性加成效果，但能加快體內SOD活化速率，來對抗體內氧化壓力。對於無規律運動習慣者，並沒有出現SOD顯著下降的激效作用情況。本研究中也再次印證規律運動可以有效提高體內SOD活性，增加人體抗氧化能力。建議未來在增補抗氧化劑的同時，應配合規律運動習慣，提高抗氧化能力的同時提早活化抗氧化酵素活性才能有效降低氧化壓力對身體造成的傷害。

關鍵字

規律運動 ； 硫辛酸 ； 超氧離子歧化? ； 氧化壓力

並列摘要

Abstract
Purpose: To investigate the influence of regular exercise and antioxidant alpha lipoic acid (ALA) supplementation on superoxide dismutase (SOD) activity in human. Methods: Subjects were divided into four groups: (1) PN group: those who took placebo and had no regular exercise habits; (2) PE group: those who took placebo and had regular exercise habits; (3) AN group: those who took ALA and had no regular exercise habits; and (4) AE group: those who took ALA and had regular exercise habits. Ten male subjects in group with a total of forty subjects. Each subject had 2 exhausted exercise tests (Bruce protocol) and between the two tests, ALA or placebo was supplied. In each exercise test, in order to analyze SOD activity in blood cells, the blood samples were collected four times: (1) before the exercise; (2) at exhaustion; (3) 15 minutes after exercise; and (4) 30 minutes after exercise. Two-way ANOVA was used for statistical analysis. Results: In the first exercise test, at exhaustion and 15 minutes after exercise, SOD activity in red blood cells of those who had regular exercise habits (PE and AE) were significantly higher than those who had no regular exercise habits (PN and AN). For the second exercise test, at exhaustion, SOD activity of PE and AE were both significantly higher than that of PN and AN. Between the two exercise tests, performances and SOD activity showed no significant differences in each group. At exhaustion of the first exercise test, AE and AN showed no significant differences (p= .079). However, in the second exercise test, there were significant differences (p= .002) and the peak value of SOD activity of AE appeared at exhaustion, instead of 15 minutes after exercise. At exhaustion, SOD activity in the first exercise test was significantly higher than that in the second test (p= .035). Conclusions: For those who had regular exercise habits, two-week ALA supplementation had no additive effects, but it effectively increases SOD activity at exhaustion to counteract with oxidative stress. For those who had no regular exercise habits, SOD does not decrease significantly. This research reassures that regular exercise can effectively increase SOD activity and antioxidant capacity in human. The suggestion is that while taking antioxidant, regular exercise habit is necessary to increase human antioxidant capacity and increases SOD activity effectively to decrease damage from oxidative stress.

並列關鍵字

regular exercise ； alpha lipoic acid ； SOD ； oxidative stress

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