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  • 學位論文

鐵鉑磁性奈米微粒之製備及其應用

Synthesis and Application of FePt Magnetic Nanoparticles

指導教授 : 陳家俊
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摘要


隨著生物醫學與奈米科技與蓬勃發展,結合材料與生物科技已經成為必然趨勢。藉由磁性物質本身的磁特性,我們可以將其與生物分子結合,而達到分離的效果。因此,近年來已有為數不少相關於磁性奈米微粒之製備及應用的文獻報導發表。 本篇論文著眼於鐵鉑磁性奈米微粒的合成。我們藉著改變微粒表面介面活性劑的組成及搭配晶種合成方式,成功的達到操控微粒尺度及均勻度之目的。此化學合成方法所合成之微粒尺度約在三奈米到七奈米之間。再者,我們調配不同比例之攜帶溶劑來稀釋所合成之磁性奈米微粒,配合微粒本身的磁特性,而呈現了高度自組裝的效果。透過此種自組裝,我們在電子顯微鏡下觀察到了單層、雙層、多層乃至於環狀的結構出現。 近年來有許多鐵鉑磁性奈米微粒與生物分子結合的文獻發表,然而此磁性材料在生物上的毒性未臻瞭解。因此,我們嘗試著將鐵鉑磁性奈米微粒修飾成水溶性並與細胞結合。利用細胞內的影像追蹤,並對此種鐵鉑磁性奈米微粒材料做細胞的毒性測試,而從目前的實驗結果初步證明,此種鐵鉑磁性材料對人類子宮頸癌細胞並不具有毒性。 最後則嘗試著結合鐵鉑磁性奈米微粒與硫化鎘半導體,此兩種功能迥異的奈米材料,利用簡便的單鍋合成方法而得到鐵鉑-硫化鎘之核-殼複合奈米材料,其尺度約十奈米左右。

並列摘要


With the proceeding of Nanotechnology and biomedicine, there is a tendency towards integrating the material science and biotechnology. We could separate the magnetic materials from the biomolecules by the characteristics of their magnetism. As a result, there are numerous literature published related to synthesis and application of magnetic nanoparticles (MNPs) up-to-now. The thesis demonstrates the organometallic synthesis of FePt MNPs. With the various surfactants and seed-mediated growth methods, we got not only tunable sizes ( about 3 nm ∼ 7 nm ) but homogeneity of as-synthesized FePt MNPs. Alternately, FePt MNPs diluting with desirable carrier solvents resulted in assembling themselves onto a solid carbon substrate. We observed the monolayer, submonolayer, multilayer and even nanoring structure in TEM images. Many applications of biomolecular-magnetic particle hybrid conjugates have been reported recently, the cytotoxicity of MNPs however has not yet been investigated. Consequently we tried to modify the FePt MNPs into hydrophilic with carboxylic acid and also investigated the toxicity of FePt MNPs in live cells using the MTT assay. And our work demonstrated that the FePt MNPs did not cause significant cytotoxicity in HeLa cells. Finally, we report on a one-pot chemical synthesis method for generating the FePt magnetic nanoparticles and the CdS quantum dots. We successfully demonstrated the conjugation of the core-shell structure of nanoparticles about 10 nm.

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