The purpose of this study was to examine the effects of exercise training combined with catechins supplementation on the antioxidant, lipid peroxidation and free radical generation in muscles injury. Forty healthy male students were voluntary to participate in this study and randomly assigned to four groups, control (C, 20.06±1.35yrs, 172.80±4.52cm, 69.50±9.85kg), catechins supplementation (S, 20.01±0.67 yrs, 171.01±5.58cm, 64.10±7.46kg), exercise training plus catechins supplementation (ES, 20.40± 1.08yrs, 172.54±5.97cm, 63.26±6.69kg) and exercise training (E, 20.50±0.85yrs, 172.01±5.91cm, 67.17± 8.2kg). Before and after treatments, all subjects performed exhaustive running test to determine VO2max, HRmax and time to exhaustion. The E and ES groups completed 4 wk running exercise training on the treadmill at the intensity of 75%VO2R (3 times per week, 20 min per session). The ES and S groups had to ingest catechins (250 mg per day) during this treatment period. The blood samples were taken from antecubital vein before, immediately and 24h after running tests to analyze the total antioxidant status (TAS), creatine kinase (CK) and malodialdehyde (MDA). The results indicated: 1) VO2max and time to exhaustion were improved in E and ES groups after training. 2) The CK activities after 24h post-running test in S, E and ES groups after training were significantly lower than in C group. 3) The TAS activity immediately after running test in ES group was significantly increased over training period, and the TAS activity after 24h post-running test in ES group after training was significantly lower than before training. 4) The MDA activity after 24h post-running test in ES group after training was significantly lower than in C group. In conclusion, the VO2max and time to exhaustion would be significantly improved after training. The activities of CK and MDA in the individuals, without exercise training experiences, would increase after intense exercise and to the peak values at the 24h post-exercise. The combination of exercise training and catechins supplementation might have a tendency to decrease the activity of MDA and to increase total antioxidant status.