芝麻、油茶與短柱山茶油體及油茶種皮之二次代謝物分析

Secondary Metabolites from The Oil Bodies of Sesamum indicum, Camellia oleifera and C. brevistyla, and from The Seed Coats of C. oleifera

葉冠宏(Guan-Hong Ye)

指導教授 ： 郭賓崇

國立虎尾科技大學/文理學院/生物科技研究所/碩士(2015年)

摘要

芝麻油與苦茶油在台灣地區被推崇為健康油脂，主要是因為芝麻油的抗氧化活性高，以及苦茶油的多元不飽和脂肪含量高等因素，然而對於種子油體中的二次代謝物則尚無完整的研究報導。故本研究選定芝麻、油茶及短柱山茶種子，進行其中所具有的二次代謝物之純化與鑑定，希望可以解析種子發芽與生長過程的機制、訊息傳遞，進而了解這些化學成分的生理活性。本研究由芝麻（Sesamum indicum）種子油體之甲醇粗萃物鑑定得到15個化合物，油茶（Camellia oleifera）種子油體之甲醇粗萃物鑑定得到8個化合物，短柱山茶（C. brevistyla）種子油體之甲醇粗萃物鑑定得到4個化合物，另外從油茶種皮之甲醇粗萃物水分畫層鑑定得到7個化合物。所鑑定得到的部分化合物，已有文獻報導其具有抗氧化與抗發炎的生理活性，顯示芝麻油與苦茶油未來具有開發成為保健食品的潛力。

關鍵字

芝麻 ； 油茶 ； 短柱山茶 ； 油體

並列摘要

The sesame oil and tea oil are appreciated as healthy oil in Taiwan, mainly due to the potential antioxidant activity of sesame oil and high content of polyunsaturated fatty acids in tea oil. However, the secondary metabolites from the oil bodies of these species were not comprehensively studied. Therefore, in the present study it was aimed to purify and identify the secondary metabolites in the oil bodies of seeds of Sesamum indicum, Camellia oleifera, and C. brevistyla. With the assistance of these results, it could construct the whole blueprint regarding the chemical constituents and physiological mechanism. It is also hopeful to elucidate the relationship between the compounds and signal transduction. Moreover, it could provide the bioactivity data of these principles. Totally, fifteen, eight, and four compounds were identified from the methanol extracts of oil bodies of S. indicum, C. oleifera, and C. brevistyla, respectively. In addition, seven compounds were isolated from the water layer of methanol extracts of kernels of C. oleifera. Some of these identified compounds were reported to exhibit antioxidant and anti-inflammatory bioactivity. These results indicated the high potential of sesame oil and tea oil as healthy food in the future.

並列關鍵字

Sesamum indicum ； Camellia oleifera ； Camellia brevistyla ； oil body

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