從阿拉伯芥(Arabidopsis thaliana)的基因功能活化品系(activation tagging line)中篩選並研究和重金屬有關的基因之功能，以及CCH在蕃茄和人類的類似基因(LeCCH、ATOX1)的功能研究

Selection of activation-tagged mutants about heavy-metal in Arabidopsis thaliana, and functional study of LeCCH and ATOX1, the CCH homolog of tomato and human.

徐碧穗(Pi-Sui Hsu)

指導教授 ： 吳少傑 ； 葉國楨

國立中央大學/理學院/生命科學系/碩士(2006年)

摘要

近年世界各國工業化程度提高，自然環境中的重金屬污染也日益嚴重，重金屬由植物吸收後對植物本身以及人類世界產生不良影響，如果可以找到植物中和重金屬影響相關的基因，並了解其作用的相關機制，對於減少環境中重金屬污染及重金屬對生物生長發育之影響將有很大助益。本研究是利用阿拉伯芥的基因功能活化品系來篩選並研究和重金屬鎘(第一章)、銅(第二章)有關的基因之功能，並利用酵母菌補償實驗(yeast complementation)和yeast two hybrid等方式研究CCH (阿拉伯芥中和植物體內銅運輸有關的基因)在蕃茄和人類的類似基因(LeCCH，第三章；ATOX1，第四章)的功能。

關鍵字

阿拉伯芥 ； 重金屬 ； 鉻 ； 銅

並列摘要

The level of industrialization of world is getting higher and higher recently, and the pollution of heavy metal in environment is getting worse. The absorption of heavy metal by plants could influence itself and human. If the gene related to heavy metal effects could be found to understand the mechanism, it is helpful for reducing the pollution of heavy metal in environment and the effects of heavy metal to the growth of living things. This research utilized the activation tagging lines of Arabidopsis thaliana to select and study the functions of gene related cadmium (chap 1) and copper (chap 2), and using methods of yeast complementation and yeast two hybrid to study the homolog of CCH (which is related to transport copper in plant) in tomato (LeCCH, chap 3) and human (ATOX1, chap 4).

並列關鍵字

arabidopsis ； activation tagging ； cadmium ； copper ； BCS ； ATX1 ； CCH ； ATOX1

參考文獻

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