光子晶體基板表面修飾銀奈米粒子增強螢光輸出研究

Silver Nanoparticles Decorated on Photonic Crystal Substrate For Fluorescence Enhancement

高悌苡(Ti-Yi Kao)

指導教授 ： 許佳振

國立中正大學/工學院/光機電整合工程研究所/碩士(2014年)

摘要

本論文結合銀奈米粒子的侷域性表面電漿共振效應(Localized surface Plasmon resonance, LSPR)以及光子晶體基板(Photonic crystal substrate, PhC substrate)的局部強場效應用來增強螢光訊號輸出。樣品製作上先是利用雙光束干涉微影技術製作一維光子晶體結構，用電子束蒸鍍機將TiO2及SiO2分別鍍在光子晶體表面，接著在表面上蒸鍍奈米厚度的銀膜，藉由退火的過程引導銀自組裝成奈米銀粒子，最後藉由牛血清蛋白(BSA-bt)形成單層的分子隔層，在樣品表面修飾Cy3螢光分子進行螢光訊號量測的實驗。利用波長532nm的雷射當激發光源，量測銀奈米粒子(Ag flat)的螢光訊號和樣品平坦區域(flat)的螢光訊號，兩者相比，由於銀奈米粒子的侷域性表面電漿共振效應，螢光增益可達5.8倍；量測光子晶體基板上修飾銀奈米粒子(Cy3-Ag coated PhC substrate)之結構的螢光訊號，和樣品平坦區域(flat)的螢光訊號比較，螢光增益14.6倍。

關鍵字

侷域性表面電漿共振效應 ； 導模共振效應 ； 銀奈米粒子 ； 光子晶體基板

並列摘要

This work, we demonstrate that the combination of the localized surface plasmonic resonance (LSPR) of silver nanoparticles and strong local filed effect of guided-mode resonance (GMR) in a resonance waveguide grating to produce surface enhanced fluorescence for bio-sensing application. The RWG was fabricated by using holographic lithography and E-gun deposition technique, and then silver nanoparticles were deposits on its surface of the grating by using thermal evaporation method. For biosensing measurement, the Cy3-tagged streptavidin bio-molecules bonded to BSA-bt layer was used as a fluorescent dye, and a 532nm solid-state diode laser was employed as an excitation beam source. The surface enhanced fluorescence can be observed up to 5.8 fold enhancement due to the LSPR of silver nanoparticles. The fluorescence can further promote when the wavelength of the excitation laser resonate with the GMR. Comparing with a flat area, the multiplicative enhancement of fluorescent signal is up to 14.3 fold.

並列關鍵字

localized surface plasmon resonance ； guided mode resonance ； silver nanoparticls ； photonic crystal substrate

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