往復式熱循環反應器之研製

Development of the Oscillatory Thermal Cycler Chamber

謝澤余(Tse-Yu Hsieh)

指導教授 ： 陳志堅

國立屏東科技大學/工學院/生物機電工程系所/碩士(2009年)

https://doi.org/10.6346/NPUST.2009.00093

摘要

論文摘要內容：本論文結合伺服馬達、8051 單晶片PWM 溫控裝置和以 铣床加工之加熱機構整合成一個往復式熱循環裝置。以PCR 所需之溫度 和反應所需之時間設計往復式熱循環裝置的加熱溫區，再以CFDRC 模擬 軟體配合副程式撰寫的邊界條件，做最佳化設計。探討反應槽於熱循環 溫區內移動速率對反應槽溫度的影響，發現反應槽移動速率為0.006m/s 時能以最快的時間，達到所需的溫度，接著探討熱循環裝置在不同條件 下進行反應槽經過熱循環實驗的結果，發現減少反應槽與加熱器間的接 觸熱阻，可有效使反應槽的溫度上升，加熱裝置的絕熱能使熱循環時的 溫度曲線更穩定，接著以不同體積的反應槽進行熱循環實驗，發現體積 較小的反應槽升降溫速率較快。比較以市售PID 溫度控制器和自製單晶 片溫控裝置加熱鋁塊進行熱循環的溫度曲線，發現兩者的溫度曲線差異 不大，這是因為當加熱溫區皆能維持穩態時，反應槽的升降溫和移動速 率有較大的關聯性，因此熱循環裝置只要能精準的控制到所需溫度並維 持在穩態溫度即可。最後驗證此熱循環機構的穩定度，結果發現經長時 間加熱後反應槽升降溫曲線和初始的反應槽升降溫曲線相似，可證明此 熱循環裝置能使溫區溫度維持一致，不受長時間加熱所影響，因此可運 用此熱循環裝置投入於長時間加熱之實驗中，如PCR 等生化反應。

關鍵字

單晶片 ； 熱循環 ； PCR ； 接觸熱阻

並列摘要

In this work we develop an Oscillatory Thermal Cycler Chamber that is
combined with the 8051 PWM controller, Smart Motor, three aluminum
heaters and chamber. To set the velocity of chamber when chamber move
through the Thermal Cycler, we use the simulation software CFDRC to find
the optimize parameters. We utilize the user subroutines compiled by
FORTRAN language to make the numerical results realistically. We find that
the effects of various moving velocities of the chamber on the temperature
distributions can be negligible. The velocity of chamber is set to be 0.0006m/s,
and three heating face can make the chamber achieve the temperature which
we need.
We investigate the temperature of the chamber which moves through
thermal cycler in different situations. Reducing the contact thermal
resistance can make temperature of the chamber higher; the heat isolation
can make the temperature of chamber more stable. Then we carry on the
experiment by using the different volumes of chambers, the heating and the
cooling rate of the small chamber is faster than the big chamber. We find that
using the heater controlled by 8051 and that by commercial PID controller to
III
heat the aluminum block, the temperature curves of the chamber are similar.
The temperatures of three aluminum heaters are at steady state, the heating
and cooling rates of the chamber are relative to the traveling speed of the
chamber, it means the thermal cycler just needs to be maintained at the
steady state temperature of aluminum block in. Finally we confirm the
stability of this oscillatory thermal cycler chamber, the temperature curve of
the chamber at the first cycle is similar to that at the specific cycle after one
hour. Therefore we may utilize this oscillatory thermal cycler chamber to
perform the PCR experiment in the future.

並列關鍵字

PCR ； PWM ； thermal cycler ； contact thermal resistance

參考文獻

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