介電泳型晶片具有操作生物細胞、細菌功能,為一種深具前瞻性的快速檢測乳品中菌種與菌含量的新方法,本論文旨在配合介電泳型晶片,開發一套可供現場快速檢測的平台,研究中利用顯微鏡的放大功能為觀察生物晶片用,配合步進馬達定位系統,可準確定位的特點,開發設計檢測儀及檢測台。針對顯微鏡的性能分析,如物鏡放大率選用,顯微鏡物鏡工作距離,顯微鏡物鏡解析度,運用ZEMAX光學模擬軟體模擬顯微鏡物鏡數值孔徑與光強度的關係,使用TracePro光學軟體模擬放置介電泳晶片情況下,對光強度的影響,及光線軌跡,另外對於步進馬達定位系統性能分析,如解析度,移動時最高速度,定位精準度,移動至同一位置的重現性
Dielectrophoretic biochip can manipulate biological cell and bacteria. Be used in dairy products rapid detection of bacteria. This thesis is intended to match dielectrophoretic biochip , developing a platform for rapid detection apply in ranch. On research use a microscope for the observation biochip and positioning system(stepper motor). Instrument the development of design and test. Microscope test: objective lens selected , objective lens work distance , objective lens resolution. Employ Zemax simulation of optical software. Simulation number aperture of objective and relationship between light intensity. Employ TracePro simulation of optical software, simulation sample is dielectrophoretic biochip, the impact of light intensity and the ray-tracing. Stepper motor positioning system for performance analysis: resolution , max speed , positioning accuracy , and location reproducibility.