This study investigated the use of IDEF0 and U-shaped production line with A company for production process improvement. According to the current status of A company, this research use IDEF0 diagrams to illustrate A’s production process of bamboo shoots, and collected the working time for each step. Against A’s production bottlenecks, this study introduced U-shaped production line to improve its production efficiency. The experimental results show that: after importing the U-shaped production line, the necessary manpower was reduced to two from three. Furthermore, according to the cost-benefit analysis, the payback period is only 3.3 years. This shows, setting up U-shaped line is really a feasible option.