酸菜醱酵液中有用微生物之分離與鑑定研究

Separation and identification of useful microorganisms from the fermention fluid of pickled mustard

方關珽(Guan-Ting Fang)

指導教授 ： 羅致逑 ； 許世興

朝陽科技大學/理工學院/應用化學系/碩士(2011年)

摘要

本論文之研究目的為增加農業廢棄物利用價值。自酸菜醱酵液中，共得 24 株分離株，經聚合酶連鎖反應 (Polymerase chain reation，PCR) 鑑定結果皆為 Lactobacillus sp.。分別對峙植物細菌性致病菌結球白菜軟腐病菌 (Erwinia carotovora subsp. carotovora，BCRC12613)、蘭花軟腐病菌(Erwinia chrysanthemi)，得到具潛力之 2 株分離株。增量培養後，2 株之分離株之上清液對兩株植物細菌性致病菌具有抑菌作用，並且對 7 株人體致病菌仙人掌桿菌 (Bacillus cereus，BCRC10446)、腸炎沙門氏桿菌(Salmonella enteritidis，BCRC10744)、鼠傷寒沙門氏菌 (Salmonella typhimurium，BCRC10747)、傷寒沙門氏菌 (Salmonella typhi，BCRC12948)、阪崎氏腸桿菌 (Enterobacter sakazakii，BCRC14122)、腸毒素型大腸桿菌 (Escherichia coli-ETEC，BCRC15372)、出血性大腸桿菌(Escherichia coli-O157:H7，BCRC15377) 也有抑菌效果。

關鍵字

抑菌作用 ； 植物細菌性致病菌 ； 人體致病菌

並列摘要

This research aims to select useful microorganisms from the fermention fluid of pickled mustard, in order to increase the value of agricultural waste. All 25 bacterial isolates are Lactobacillus sp. . Compare antibacterial activity of the plant pathogens bacterial Erwinia carotovora subsp. Carotovora (BCRC12613), Erwinia chrysanthemi, we choosed the 2 from the potential isolates. By culturing the 2 isolates and using cell-free supernatant for antibacterial activity plant pathogens bacterial. There are antibacterial activity from human pathogens bacterial: Bacillus cereus(BCRC10446), Salmonella enteritidis(BCRC10744), Salmonella typhimurium(BCRC10747), Salmonella typhi(BCRC12948), Enterobacter sakazakii(BCRC14122), Escherichia coli-ETEC (BCRC15372), Escherichia coli-O157:H7 (BCRC15377).

並列關鍵字

plant pathogens bacterial ； antibacterial activity ； human pathogens bacterial

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