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  • 學位論文

無患子環保清潔劑產品與分析方法的開發

The Development of Sapindus environment-friendly Cleanser and its Analytical Method

指導教授 : 許世興
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摘要


由近年來,由於化學合成清潔劑對生活環境中的破壞,不僅汙染了水源,而且還無形的產生致癌的機率,對於人體與自然環境中的影響也是日積月累的。而在化工肥皂還有沐浴商品還未開發前,無患子果實就是以前老一輩人做為清潔的天然洗潔劑。無患子本身含有皂苷素能產生泡沫且溶於水,無患子是一種天然的非離子型的界面活性劑,界面活性強,低濃度就可以發泡,進入水溝後能夠被發酵分解,是具有環保的天然洗潔劑。 本研究是建立一套新的 HPLC 分析方法來檢測無患子產品中無患子皂素群成分的穩定性,移動相為水:乙晴,其比例為 50:50,連接 ODS Hypersil 250×4.6 mm(5μm) 的層析管柱,偵測波長為 206 nm,流速為 1.0 mL/min,注射量為 10μL,此分析條件可在10分鐘內完成。 在分析確效方面,自備型純化無患子標準品其線性迴歸係數R2 為 0.9997;。代表精密度的相對標準偏差(RSD)介於0.04 %~1.26 %之間。代表準確度的回收率為99.35 %。 在無患子產品的試驗方面,加入少量的防腐劑及發泡劑的無患子萃取液,實驗觀測結果而在49~63天之後都會產生發霉與沉澱現象,而皂素群成份波峰面積的變化量增加。而改良後兩種無患子產品:無患子洗手乳與無患子洗廁劑,因為加入了具有抗菌與殺菌功能的香茅精油,使兩種產品在六個月期間無發現發霉現象,安定性相當理想。

並列摘要


In recent years, the chemically synthetical cleansers not only pollute the water resources, but also increase the probability of human carcinogenicity. Such destroying impacts to humans and environment have being accumulating. Before developed of these chemical soaps and industrial bathing detergents, Sapindus had being used as a natural cleanser for very long time. Sapindus contains abundant saponins, which are water-soluble and can produce foams. Sapindus is a non-ionic surfactant as well as strong interface activity and can be foamed with low concentration. It can be easily decomposed by fermenting after flowed into the gutter and is a natural green detergent. In this research, a HPLC method was developed to determine the ingredients of saponins in Sapindus. The mobile phases were prepared with water and acetonitrile with the ratio of 50 to 50. Chromatographic methods were performed with a HPLC system equipped with ODS Hypersil 250×4.6 mm(5μm) column with UV/Vis absorption set at 206 nm, flow rate set at 1.0 mL/min and an injection of 10 μL as well as each runtime about ten minutes. In the result of analytical method validation, the linear regression coefficient (R2) was 0.9997, the relative standard deviations(RSD) of precision experiments were between 0.04 %~1.26 %, and the recoveries of accuracy experiment were 99.35 %. In the observation tests of Sapindus products by adding a small amount of preservative and foaming agent to Sapindus extract, the experimental products all became moldy and precipitated within 49 to 63 days and the peak area of saponin group ingredients increased in the period of the experiment. After plenty of tests, we developed two Sapindus products: Sapindus hand cleanser and Sapindus toilet spray detergent. After the adding of citronella oil with the antibiotic and disinfecting capacity, there was no mold found in these two products for six months with relatively good stability.

參考文獻


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