Due to rapid social change and transformation of the educational environment, the work stress of elementary school teachers is more than before. This not only affects the mental load and physical condition of teachers, but also affects the educating rights of children. The goal of this research is to assess the performance of music therapy for the different work load on the elementary school teachers with the heart rate variability. There were 31 participants who all are the elementary school teachers. The heavy work load is defined as the case that teachers have more than four classes in one day. The light work load is defined as the case that teachers have less than two classes in one day. The participants all listened the Wind Music and Hayley Music for 20 minutes on the different work load. The results indicated when teachers listened the Wind Music under a heavy work load, the parameters of their heart rate variability have the significant difference.