樟芝(Antrodia cinnamomea),為台灣特有的野生真菌,生長於台灣的特有牛樟樹(Cinnamomum kanehirae)椴木上,其子實體生長速度非常緩慢,子實體中含有許多天然小分子化合物,其中的天然物4-acetylantroquinonol B(4-AAQB),為此研究主要的重點,4-AAQB化合物對肝癌細胞HepG2具有抑制增殖活性之作用,本研究以液態發酵的方式生產4-AAQB,在有限空間之下,可大量生產發酵液,同時探討樟芝以液態發酵過程中在發酵液中4-AAQB的含量,本研究利用液態發酵生產方式,以提高4-AAQB 產率為目標,再將發酵液以乙酸乙酯(EtOAc)萃取,以MALDI-TOF/MS快速分析以確認發酵液中4-AAQB含量,並進一步開發定量方法,初步結果皿培已經得到與椴木顏色相符之類子實體,液態發酵生產4-AAQB四種條件,則以C條件為最佳, 可得到較佳的4-AAQB含量。
Antrodia cinnamomea is an unique wild fungus in Taiwan, which can only grow on the unique Cinnamomum kanehirae basswood. Its fruiting body grows very slowly. In this study, we focus at production of 4-acetylantroquinonol B (4-AAQB) which is a natural compound containing in A. Cinnamomea. 4-AAQB has been reported of anti-proliferative activity on hepatoma cell HepG2. In this study, a submerged cultivation of A. Cinnamomea was performed. Th ethyl acetate (EtOAc) extract of the fermentation broth was analyzed using MALDI-TOF/MS to identify the existence of 4-AAQB in fermentation broth. Further quantification method is now under development. Preliminary results have been consistent with dish culture of basswood color category fruiting bodies, Liquid fermentation 4-AAQB four kinds of conditions, C condition are the best available preferred 4-AAQB content.