以 MALDI-TOF 質譜法快速鑑定不同牛樟芝三?類成分

Fast analysis of triterpenoids from Antrodia camphorata by MALDI-TOF MS

林彥良(Yan-Liang Lin)

指導教授 ： 曾耀銘 ； 陳政男

朝陽科技大學/理工學院/應用化學系生化科技碩士班/碩士(2013年)

摘要

牛樟芝萃取出的三?類化合物 (triterpenoids) 具有很好的生物活性，進行抗癌、抗發炎、降血壓、降血脂及活化神經細胞等功效，在亞洲廣泛運用在機能性食品及藥物發展。牛樟芝麥角甾烷 (ergostane) 三?類化合物是真菌中較為罕見，可作為鑑定牛樟芝的指標成份。目前市面上牛樟芝產品來源大致來自於液態培養或固態培養，所含三?種類也不同。本實驗利用開發檢測三?類成分之方法。在此研究中搭配2,5-DHB利用十八烷基三氯矽烷 （octadecyltrichlorosilane，OTS) 修飾鋁箔來形成表面疏水性，使用在鋼盤上可以有效提高分析物訊號強度做為基質可以鑑定出包含8種麥角甾烷化合物: Methyl antcinate B (AC 1) 、Antcin B (AC 5) 、Antcin H (AC 7) 、Antcin A (AC 9) 、Antcin C (AC 10) 、Methyl antcinate A (AC 11) 、Antcin K (AC 12) 、Methyl antcinate H (AC 13)，建立出AC 1、AC 5、AC 7、AC 9、AC 10、、AC 11、AC 12、AC 13 檢量線，其線性範圍 2~10 ppm (R >0.99)。分析出液態培養和固態培養的牛樟芝內三?類成分，發現子實體含有八個麥角甾烷，而菌絲體只有AC 7、AC 10、AC 13，區分出菌絲體及子實體差異性。

關鍵字

MALDI-TOF MS ； 三?類 ； 牛樟芝

並列摘要

Antrodia camphorata (AC) is a unique mushroom of Taiwan. It parasite on the protected endemic species Cinnamomum kanehirae (Bull camphor tree).The extracted triterpenoids from A. camphorata express excellent bioactivities, like anti-cancer, anti-inflammatory, lowering-blood pressure, lowering-blood lipids and activate nerve cells and other effects. It was widely used in functional (health) foods and medicine development in Asia.
Currently, the products of A. camphorata on market come from different sources. Based on liquid culture or solid-state cultured condition, the triterpenoid components are also different. Usually, triterpenoids was analyzed by HPLC, LC-MS/MS was also used to do pharmacokinetic study of triterpenoids in rat blood. No study reports the application of MALDI-TOF MS on analysis of A. camphorata. In this study, a fast, sensitive and enviromental friendly analysis method by MALDI-TOF MS will be applied to determine eight triterpenoid componds of A. camphorata. A octadecyltrichlorosilane (OTS) modified hydrophobic aluminum foil surface was used on target plate to increase the analyte signal intensity. The identified analytes include methyl antcinate B (AC 1), antcin B (AC 5), antcin H (AC 7), antcin A (AC 9), antcin C (AC 10), methyl antcinate A (AC 11), antcin K (AC 12), and methyl antcinate H (AC 13). The results, showing the linear range and r2 value are 2 ppm~20 ppm with r2 = 0.954 ~ 0.996, respective. This method will be able to fast distinguish the different sources of A. camphorata products on market.

並列關鍵字

Antrodia camphorata ； triterpenoids ； MALDI-TOF MS

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