透過您的圖書館登入
IP:3.234.177.119
  • 學位論文

乳酸菌的活性對於其在Caco-2與HT-29細胞株黏附的影響

Effects of viability of lactic acid bacteria on adhesion to Caco-2 and HT-29 cell-line

指導教授 : 顏聰榮
若您是本文的作者,可授權文章由華藝線上圖書館中協助推廣。

摘要


部分文獻指出,死去的益生菌依然可以黏附在組織培養的細胞上,這似乎暗示了存活、活體並非益生菌黏附作用所必須。另項研究觀察Lactobacillus GG與L. rhamnosus LC-705經過熱與酸的處理後,可提高其對黃麴毒素的結合能力,其他像UV光照、醇、鹼等處理皆無法影響其結合能力,這代表了結合能力的消長原因可能是因為細胞壁外層結構改變,而非菌種的生長能力改變。近期藉由原子力顯微鏡的測試觀察結果顯示:乳酸菌的黏附能力與其細胞壁表層結構成分有密切關係。 本研究培養幾種黏附力強弱程度不同的乳酸菌,觀察其在正常生長狀態下、自然失去生長能力狀態下(因營養條件不足,導致失去生長能力的菌體,將細胞壁外層結構可能變化的機率降至最低)以及非接觸性外力介入失去生長能力狀態下(UV照射24小時後的菌體,破壞其代謝功能而不影響細胞壁外層結構)的黏附力。比較結果發現不同的生長能力狀態下,各種受測乳酸菌對於相同的細胞組織(Caco-2或HT-29)之黏附力並沒有非常顯著的差別,而對不同的細胞組織(Caco-2與HT-29)之黏附力仍有類似的趨勢可比對,而且連菌體破片也依然保持類似於完整菌體的黏附密度。由此種種說明了不同種類乳酸菌的黏附能力與其活性無關,只與其細胞壁表層結構成份有關,並確認乳酸菌的存活並非是腸道黏附的重要因子。

關鍵字

活性 黏附 乳酸菌 益生菌

並列摘要


Several studies have reported that non-viable probiotics are able to adhere to tissue culture cells indicating that viability is not necessary for adhesion of probiotics. Other study has been observed that heat and acid treatment of Lactobacillus GG and L. rhamnosus LC-705 enhances the binding capacity of these strains for aflatoxins. Other treatments, such as UV-irradiation, or treatment with alcohol or alkali, did not affect the binding capacity. This suggests that possible changes in the structure of the cell envelope, rather than inactivation of the strains, are responsible for the enhanced binding. During the last years, AFM(atomic force microscopy) study has been observed that there is close relation between adhesion forces of LAB(lactic acid bacteria) and LAB cell wall constituents. This study was to culture several LAB of different adhesion forces, and observed adhesion forces change between normal viable state, nature non-viable state(nature death because no nutrient supplement, reduce risk of change of cell wall constituents) and forced non-viable state(UV-irradiation 24 hours, destroy metabolism function and kept cell wall constituents completely). The result compared different viable state observed that adhesion forces is no obviously change in every strain of LAB adhere to same cell model(Caco-2 or HT-29), and similar adhesion forces trend in every strain of LAB adhere to different cell model(Caco-2 and HT-29), and even bacterial fragment still keep same adhesion density as completely bacteria. Therefore we have proved the adhesion force of LAB has no relation with bacterial viability, but cell wall constituents. We have confirmed the survival of LAB was not important factor on adhesion to human intestine cells.

並列關鍵字

adhesion Caco-2 HT-29 LAB probiotic viability

參考文獻


Yi-Tang Chang (2004). Influence that the environmental factor microorganism grows -UV irradiation. Microbiology-Selected Experiments, 361-362.
Chi-Cheng Liao (1998). Classification and application of lactic acid bacteria. Food Industry, 30: 1-10.
Chi-Lin Tsai (2002). Isolation and identification of lactic acid bacteria from fermented vegetables and analysis for use as probiotics. THESIS of Tatung University
Akkermans, A. D. L., Zoetendal, E. G., Favier, C. F., Heilig, H. G. H. J., Akkermans-Van Vliet, W. M., & De Vos, W. M. (2000). Temperature and denaturing gradient gel electrophoresis analysis of 16S rRNA from human faecal samples. Biosci. Microflora, 19, 93-98.
Alander, M., Korpela, R., Saxelin, M., Vilpponen-Salmela, T., Mattila-Sandholm, T., & Von Wright, A. (1997). Recovery of Lactobacillus rhamnosus GG from human colonic biopsies. Lett Appl. Microbiol. 24, 361-364.

延伸閱讀