利用稀硫酸搭配冷電漿表面修飾技術增進 SPR光纖感測器之靈敏度

THE ENHANCEMENT METHOD OF SPR FIBER BIOSENSOR WITH ACID AND PLASMA SURFACE MODIFICATION

趙敏孜(Min-tzu Chao)

指導教授 ： 蔡五湖

大同大學/電機資訊學院/光電工程研究所/碩士(2009年)

摘要

光纖生物感測器具有體積小、功率低、重量輕、高效能等特性，並且能抗惡劣環境、無輻射干擾、及頻寬大、容易實現多工多點分佈式感測系統等優點，同時對於待測生物體、臟器、組織、或生化物質，具有無毒性與高度的生物相容性，能耐受體液的長期腐蝕、不凝血、不溶血、無生物排斥性的影響，因此光纖感測器極適合用於生物感測上，同時在生命科學與生醫應用上受到普遍的重視與興趣。
本研究的重點在於表面電漿共振光纖生醫感測技術之創新開發，以側磨型漸逝波光纖SPR 感測器為主，配合表面鍍金層及冷電漿的表面改質方式取代傳統所使用MUA (11-mercaptoundecanoic acid)浸泡的表面改質方法，同時由於羧基(COOH¯)可以和生物分子作一強力之鍵結，因此在冷電漿表面改質中，我們選擇使用解離後含有羧基(COOH¯)的IPA (isopropanol)電漿，並且提出在鍍金前使用酸處理(濃度為10%的H2SO4)製程清潔表面，增加金膜和表面的鏈結度以及和生物分子之結合力的表面改質方法，除了可將反應時間由原本的一天,縮短為不到2小時之外，對於表面均勻度以及和生物分子的結合力有顯著的改善,同時亦大幅的提升偵測生物的靈敏度。

關鍵字

表面電漿共振 ； 冷電漿 ； 表面改質 ； 光鮮感測器

並列摘要

Fiber optic sensors for bio-chemical measurement, with such excellent properties as small size, low power, low weight, low cost, having potential for real-time detection, being disposable, being free of labeling, good performance, environmental ruggedness, and immunity to electromagnetic interference, as well as the advantages of large bandwidth and ease in implementing multiplexed or distributed sensors. In the mean while fiber optic sensors are nontoxic and high compatible with living creature, internal organs, tissues, and biochemical materials, as well as both materials can endure the corrosion from body fluid and compatible with blood, so that the fiber optic sensors have the superiority using in the biochemical measurements, also would have been of interest and importance in life sciences and biomedical applications.
In this thesis, the major work is concentrated on the development of optical fiber biosensors based on surface plasmon resonance technology. We use multi-mode side-polished fiber sensor with surface gold metal coating and cold plasma modification technique replace the traditional modification way of soaking 11-mercaptoundecanoic acid (MUA).
We choose isopropanol (IPA) plasma to increase carboxyl group on side-polished surface of the optical SPR fiber biosensor we proposed, and they are easily to combine biomolecule by EDC/NHS modification. Before IPA plasma treatment, we use acid pre-treatment (H2SO4 10%) solution in order to clean the surface before gold thin film coating to enhance the bonding forces between fiber surface and gold thin film with significant enhancement results on biosensing. The surface modification proposed in the thesis not only can decrease the reaction time from 24hours to less than 2 hours, but also can obviously improve the surface uniformity and sensitive and biocompatibility of the side-polished SPR fiber sensor.

並列關鍵字

acid treatment ； SPR ； cold plasma ； fiber biosensor

參考文獻

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