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  • 學位論文

製備熱敏感性幾丁聚醣磁性水膠應用於癌症熱治療

Preparation of Thermosensitive Chitosan Ferrogel for Cancer Hyperthermia

指導教授 : 周澤川
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摘要


癌症已為國人十大死因之一,針對於癌症的治療亦是生物醫學領域重要指標。環境應答高分子中,以溫度敏感性水膠尤受矚目,利用相轉移變化所形成之膠體既簡單又安全並具生物可溶性與分解性,因此在人體生醫應用相當廣泛。磁力為一種非侵入性且對於人體不會造成傷害,運用磁性奈米粒子在高頻變化磁場下產生磁性消散並釋放出熱能應用於磁熱治療上,是一特殊技術。將磁性流體注射至患部易被血液快速流動而易被代謝,故將磁性奈米材料結合智慧型水膠製備磁性-溫度敏感性水膠,針對腫瘤細胞進行磁熱治療之應用。 以幾丁聚醣結合Glycerophosphate合成溫度敏感性水膠使其於人體溫度下能快速應答,由研究結果顯示在37℃下在25±2秒可由溶液轉變為膠體,並在膠體中加入經葡萄糖酸內酯(Glucono--lactone)修飾之氧化鐵磁性流體,利用高週波電感應加熱器測試磁性凝膠之升溫能力,濃度為35mg Fe3O4/ml磁性-溫度敏感性水膠,可於10分鐘升溫達91.9℃。取此濃度磁性水膠注入體外組織,其升溫效果為20分鐘內,溫度變化量為29.3℃(△T=29.3℃)。將磁性-溫度敏感性水膠注入大鼠肝臟,於48小時後經切片、鐵質染色,可發現磁性-溫度敏感性水膠較磁性流體易存留於體內組織。磁性-溫度敏感性水膠之特性分析,以X-ray繞射儀(XRD)分析結構與晶相,利用傅立葉轉換紅外線光譜儀(FT-IR)、熱重分析儀(TGA)鑑定水膠確實包覆於磁性奈米粒子外層,並以穿透式電子顯微鏡(TEM)觀測水膠包覆磁性奈米粒子情形,估算粒徑約為22-34nm,並利用超導量子干涉儀(SQUID)測量磁性奈米粒子、磁性-溫度敏感性膠體之飽和磁化量。 本研究所製備之磁性-溫度敏感水膠能有效地在人體溫度下(37℃)快速成膠且具備良好升溫效果,達到磁熱治療之目的。

並列摘要


Cancer has been one of the leading causes of death for the people and the treatment of cancer is an important indicator of the biomedical field. Environmental response polymer such as thermosensitive hydrogel is attractive phenomena. The phase transfer of colloidal material is simple, safe and biodegradable, and therefore it can be applied for human biomedical in wide range. Magnetic field is a non-invasive and will not cause harm to the human body, and the ability of magnetic nanoparticles can be used as heat source of hyperthermia in the high frequency magnetic field which releases magnetic energy to perform magnetic hyperthermia. In order to avoid ferrrofluid was easily metabolized by the rapid flow of blood , and kept in tumour for desured period.Consequently, the magnetic nanoparticle prepared by combining smart hydrogel to synthesize magnetic-thermosensitive hydrgel (ferrogel) in connection with the tumor cells for the application of hyperthermia. In this research, synthesis of the ferrogel with chitosan and quaternized chitosan (HTCC) containing. Glycerophosphate which can quickly interaction with tissue in human body. The result indicated the prepared ferrogel can be transformed into gelation for 25 ± 2 sec. at 37 ℃. Addition of ferrofluid which contains modified with glucono--lactone to ferrogel in the tumor is good method for hyperthermia. The heat ability of ferrogel were tested by commercial high frequency machine. In 10 minutes, magnetic-thermosensitive ferrogel can get 91.9℃ which concentration is 35mg Fe3O4 per ml ferrogel. Inject identical concentration ferrogel in vitro. The temperature could change 29.3℃ in 20 minutes. After 48 hours, we could find the ferrogel that injected to rat’s liver. The ferrogel were easily retained in vivo than ferrofluid. Through this formula, the synthesis of ferrogel can be effectively and quickly generated heat in the human body and has a good heating effect to reach the hyperthermia.

並列關鍵字

thermosensitive hydrogel hyperthermia Fe3O4

參考文獻


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被引用紀錄


邱文鑑(2016)。溫感性奈米碳管複合水膠於磁性與近紅外光加熱效用之研究〔碩士論文,中原大學〕。華藝線上圖書館。https://doi.org/10.6840%2fcycu201600312

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