多孔生醫玻璃陶瓷支架之製作

Fabrication of porous bioglass-ceramic scaffolds

李宗品(Tsung-Pin Li)

指導教授 ： 吳世經

中臺科技大學/健康科學院/醫學工程暨材料研究所/碩士(2008年)

摘要

三維的多孔生醫玻璃陶瓷支架在骨缺陷填補上扮演一重要角色。因複製法所製作的支架相通性、孔隙???好，因此，本研究使用複製法製作多三維生醫玻璃陶瓷支架，粉末採用由Hench 所研發之45S5 玻璃粉末以及本實驗室發展之MgO-CaO-Al2O3-SiO2-P2O5 玻璃陶瓷粉末製作。除?測?機械性質外，並將材?與骨母細胞 (MG-63)共培養後進?MTT 分析細胞存活?，並以掃描式電子顯微鏡觀察細胞貼附之?況。實驗結果顯示，批覆?漿?想比?為，二次水：100 ml ， PVA ： 5 克， 玻璃粉末： 50 克。細胞培養的部分，MgO-CaO-Al2O3-SiO2-P2O5 的細胞??在塊材或多孔支架的貼附效果?好，45S5 塊材因材?釋放?子，使培養液的pH 值上升，細胞?貼附、生長。MgO- CaO-Al2O3-SiO2-P2O5 製作之支架抗壓強?比45S5高，海綿孔隙尺寸對抗壓強?的影響?大，影響抗壓強?的最大因素是孔隙?。

關鍵字

生物相容性 ； MG-63 ； 複製法 ； 生醫玻璃陶瓷 ； 多孔支架

並列摘要

Three-dimensional porous bioglass-ceramic scaffolds played an important role in bone defect. Replication technique could fabricate scaffolds interconnect well and high porosity. The glass powder 45S5 and MgO-CaO-Al2O3-SiO2-P2O5 were developed by Hench and the laboratory respectively. The biocompatibility was evaluated by means of the MTT test. The cell adhesion was observed by SEM. An optimum slurry ratio was deion water : 100 ml, PVA : 5 g, glass powder : 50 g. In the cell culture, the MG-63 was adhesion well whether on MgO-CaO-Al2O3-SiO2-P2O5 bulk or scaffolds. Bioactive glass 45S5 is used with superior effect in vivo, testing of the same material could be
adverse to cells because of pH increases over the buffered range of medium in vitro. Scaffolds fabricated by MgO-CaO-Al2O3-SiO2-P2O5 had higher compressive strength than 45S5. The factor affected compressive strength was porosity mainly.

並列關鍵字

Biocompatibility ； MG-63 ； Compressive strength ； Replication technique ； Bioglass-ceramic ； Porous scaffolds

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