經微波萃取鴻喜菇和美白菇之抗氧化活性

Antioxidant properties of Hypsizigus marmoreus and the white mutant of Hypsizigus marmoreus by microwave extraction

王世敏(Shi-min Wang)

指導教授 ： 李煜玲

中臺科技大學/健康科學院/食品科技研究所/碩士(2012年)

摘要

本研究係針對鴻喜菇［Hypsizigus marmoreus, Peck. Bigelow, HP］與美白菇（white mutant of Hypsizigus marmoreus, WHP）子實體為實驗材料進行萃取，以不同時間熱水及微波萃取（10、30及60分鐘），以及熱水（對照組）和不同微波功率處理（100、200、及400 W）後再使用熱水萃取，並將所得萃取物，進行多醣含量、多醣分子量、抗氧化成分及抗氧化活性分析。
在不同加熱時間下，鴻喜菇（HP）與美白菇（WHP）經熱水萃取（HWE）及微波萃取（MWE），隨萃取時間增加萃取率有下降趨勢，萃取率以熱水萃取高於微波萃取。HP與WHP的總醣含量會隨熱水萃取時間增加而增加。反之，微波萃取則隨萃取時間增加而降低。在多醣分子量分析上，HP熱水及微波萃取30分鐘後，其多醣分子量最大分別為19.6 × 103 Da及23 × 103 Da；WHP隨萃取時間增加多醣分子量也越大16.7 × 103 Da ~27 × 103 Da。在抗氧化性質方面，HP與WHP分別經熱水萃取30分鐘後，濃度在10 mg/mL時抗氧化力最高，分別為74.85及83.42%，而微波萃取隨時間增加抗氧化力有增加趨勢，HP與WHP在濃度10 mg/mL，萃取時間60分鐘，其抗氧化力為88.21及87.90%，整體而言，在不同時間下微波萃取物之抗氧化力比熱水萃取高。在不同時間下，熱水與微波萃取HP皆以萃取60分鐘所得之萃取物還原力最高（0.59及0.84）；WHP熱水萃取物隨萃取時間的增加還原力也隨之提高，當濃度10 mg/mL時，還原力由0.77增加至0.82，若WHP在相同濃度下以微波萃取30分鐘其萃取物，還原力最高為0.79。在清除DPPH自由基能力方面，HP與WHP經不同時間熱水與微波萃取後，在10 mg/mL下，微波萃取清除DPPH自由基能力（42.73~55.65）方面，較熱水萃取（39.96~46.83%）高。在捕捉羥自由基能力，濃度20 mg/mL下，HP和WHP皆以熱水效果高於微波萃取。在螯合亞鐵離子能力方面，HP與WHP經不同時間熱水與微波萃取，濃度在10 mg/mL下螯合能力都高達95%，因此，熱水及微波萃取，皆讓此兩種菇具有良好螯合能力。在抗氧化成分方面，WHP抗壞血酸含量比HP高，HP以熱水萃取10分鐘即測得抗壞血酸0.03 mg/g；WHP在熱水萃取物與微波萃取物，其抗壞血酸含量高達0.06~0.11 mg/g及0.48~0.54 mg/g。在總酚類化合物含量上，以微波萃取HP 10分鐘後其含量最高7.88 mg/g，WHP則以熱水與微波分別萃取10分鐘，分別達10.35及10.36 mg/g。在麥角固醇含量上，以不同時間熱水萃取HP為3.26~3.58 mg/g，WHP分別以熱水萃取60分鐘及微波萃取30分鐘時含量最高，分別為4.37及4.31 mg/g。
另外，經不同微波功率處理後，HP與WHP隨功率增加萃取率跟著提高，其中以400 W萃取率最接近熱水萃取46.87及51.57%。總醣含量則以100 W微波萃取含量最高24.22及25.74%。HP與WHP萃取物之多醣分子量也隨功率增加而越大，其分子量範圍23 × 103~51.3 × 103Da，且WHP大於HP。抗氧化力分析上， HP經不同微波功率萃取，在20 mg/mL下（95.75~96.66%），較熱水萃取（94.34%）高，WHP在不同微波功率萃取下，濃度為5 mg/mL時，隨功率增加抗氧化力有增加的現象，分別為45.80、51.48及至54.09%，在相同濃度熱水萃取為48.24%。在濃度為10 mg/mL時，還原力隨功率增加有增加的趨勢，HP分別為0.72、0.80及0.83，而WHP為0.86、0.86及1.03。在清除DPPH自由基能力方面，當濃度在20 mg/mL時，隨者微波功率增加，其清除率有下降的趨勢，HP清除率由83.13降至78.73%。相反的，WHP在相同濃度下，則隨瓦數增加清除率有提高的現象，由78.51提高到85.92%。HP在微波200 W，濃度20 mg/mL下，具有較佳的捕捉羥自由基能力為43.05%，WHP則為400 W較高（40.76%）。HP與WHP濃度20 mg/mL經不同微波功率萃取物之螯合能力皆高達99%。HP與WHP在不同微波功率下抗壞血酸含量為0.02~0.07 mg/g。HP隨微波功率增加總酚含量也有提高，（14.54提高到15.70 mg/g），但熱水萃取含量則仍最高（16.16 mg/g），另外，WHP以微波400 W含量最高15.76 mg/g。HP之麥角固醇含量微波功率以200 W含量最高（4.92 mg/g），WHP以100 W含量最高（2.80 mg/g）。
綜合以上結果，HP與WHP經由不同時間熱水及微波和不同微波功率對萃取物皆有不同程度之抗氧化功能。其中，在不同時間下，微波萃取可增加HP與WHP中總醣、麥角固醇及總酚含量與抗氧化力；另一方面，隨著功率增加，HP與WHP抗氧化、還原及螯合之能力明顯提高，只有HP清除DPPH自由基能力增加，由此推論，微波萃取食用菇，可作為食用菇一項新萃取技術的選擇。

關鍵字

微波萃取 ； 抗氧化性質 ； 美白菇 ； 鴻喜菇

並列摘要

This research used Hypsizigus marmoreus (Peck.) Bigelow, white mutant of Hypsizigus marmoreus fruit bodies to study two parts, the first part is used of various times of hot-water-extracted and microwave extracted, and the second part is used of various watts of microwave extracted. Then, to evaluate the polysaccharide content, polysaccharide molecular wight analysis and antioxidant properties of those extracts.
In the various time, the extraction yields of H.marmoreus hot water extracted is the highest as 48.98 % after 10 minutes heating, and that of white mutant of H. marmoreus is 43.31%. In the total sugar content, H.marmoreus and white mutant of H. marmoreus microwave extracted are higher as 33.28 and 31.83% after 10 minutes heating. Using gel filtration, the molecular weights of hot water and microwave extracted from H.marmoreus after 30 minutes heating are higher as 19.6 × 103 and 23 × 103 Da. The molecular weights of polysaccharides from white mutant of H. marmoreus are increased with heating time increasing and those are range of 16.7 × 103 ~27 × 103 Da. Using the conjugated diene method, the hot water extracted from H. marmoreus and white mutant of H. marmoreus after 30 minutes heating are higher antioxidant activity at 10 mg/mL and those are 74.85 and 83.42%, respectively. At 10 mg/mL, the microwave extracted from H. marmoreus and white mutant of H. marmoreus after 60 minutes heating are higher as 88.21 and 87.90% , respectively. The reducing powers of hot water and microwave extracted after 60 minutes are 0.59 and 0.84 at 10 mg/mL for H. marmoreus, respectively. The microwave extracted from white mutant of H. marmoreus after 30 minutes heating is 0.79 at 10 mg/mL. The scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl radicals of the hot water and microwave after various time heating from H. marmoreus are the range of 42.73~55.65% at 10 mg/mL. The chelating ability on ferrous ion, hot water and microwave extracted form H. marmoreus and white mutant of H. marmoreus are to reach 95% at 10 mg/mL.
In antioxidant components, ascorbic acid contents of the hot water extracted and microwave extracted after 30 minutes heating from H. marmoreus and white mutant of H. marmoreus are the range of 0.06~0.11 mg/g and 0.48~0.54 mg/g. In total phenols content, microwave extracted after10 minutes heating from H. marmoreus is the highest as 7.88 mg/g. The hot water and microwave extracted after 10 minutes heating from white mutant of H. marmoreus are higher as 10.35 and 10.36 mg/g, respectively. In addition to ergosterol contents, the hot water and microwave extracted after 30minutes from white mutant of H. marmoreus are 4.37 and 4.31, respectively.
The extraction yields of H.marmoreus and white mutant of H. marmoreus hot water extracted is the highest as 54.45% and 52.05%, followed 400 watts 46.87% and 51.57%, respectively. total sugar contents of H.marmoreus and white mutant of H. marmoreus at 100 watts microwave extracted are 24.22 and 25.74%. The molecular weights of polysaccharides from H. marmoreus and white mutant of H. marmoreus used various watts microwave extracted are the range of 23 × 103 ~51.3 × 103 Da. Using the conjugated diene method, the antioxidant activities of various power of microwave extracted from H. marmoreus are 95.75~96.66% at 20 mg/mL. Then, the microwave extracted at 100, 200 and 400W from white mutant of H. marmoreus are 45.80, 51.48 and 54.09%, respectively. The reducing powers of 100, 200 and 400W microwave extracted are 0.72, 0.80 and 0.83 at 10 mg/mL for H. marmoreus and 0.86, 0.86 and 1.03 for white mutant of H. marmoreus, respectively. In addition, the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals of H. marmoreus are dreased with the power increasing. The various power extracted from H. marmoreus and white mutant of H. marmoreus after are higher chelating abilities on ferrous ion and are to reach 99% at 10 mg/mL. The total phenol contents of various power for H. marmoreus and white mutant of H. marmoreus are the range of 13.82~15.76 mg/g. In the ergosterol contents, H. marmoreus at 200 power t is the highest as 4.92 mg/g and white mutant of H. marmoreus at 100 power is the highest as 2.80 mg/g.
Overall, H. marmoreus and white mutant of H. marmoreus are used various times and watts extracted, their hot water of microwave extractions exhibit various levels of the antioxidant properties. Total sugar, ergosterol, total phenol and antioxidant activity of microwave extracted by various times for HP and WHP are increased. In addition, antioxidant activity, reducing pwoer and chelating ability of microwave extracted by various watts are increased. Then, the results show that microwave extraction method maybe used as a new choice.

並列關鍵字

H. marmoreus ； white mutant of H. marmoreus ； antioxidant properties ； microwave extracted

參考文獻

黃振哲，2010。食用菇類不同多醣體之製備與其抗氧化活性評估。中臺科技大學食品科學系碩士論文。台中，台灣。

Angelo, A. J. S.. 1996. Lipid oxidation in foods. Critical Reviews in Food Science and Nutrition 36(3): 175-224.

Antioxidant activity of microwave-assisted extract of Buddleia officinalis

Bartnicki-Garcia, S. 1968. Cell wall chemistry, morphogenesis and taxonomy of fungi. Annu. Rev. Microbiol. 22: 87~108.

Brand-Williams, W., Cuvelier, M. E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT- Food Science and Technology 28:25-30.