磁場對纖維母細胞遷移的影響

Study on the effects of magnetic field on the fibroblasts cell migration

張佳琪(Chia-Chi Chang)

指導教授 ： 張振榮

中臺科技大學/健康科學院/醫學影像暨放射科學系暨研究所/碩士(2012年)

摘要

近幾年來，磁性奈米材料運用於細胞追蹤已成為一種趨勢，然而並未有研究探討將細胞與磁性奈米物質培養並利用磁場驅動細胞遷移。因此本研究將加入Gd及核磁共振造影儀所產生之磁場評估對於細胞遷移的影響。
L929纖維母細胞以含10%胎牛血清之H-DMEM培養基進行培養，待細胞貼附與生長到一定密度，加入含有Gd-DOTA培養基培養。24小時後，將細胞置於磁場環境下，以每小時刺激10分鐘，至6小時(間斷式)；或連續刺激1小時/放置5小時（連續式）。
MTT、NO、LDH結果皆顯示Gd-DOTA(15%以下)於體外研究中可使細胞穩定生長其細胞毒性極低。細胞遷移結果顯示成效最好之組合為：間斷式刺激－Parallel磁場，而Parallel磁場中以不添加Gd-DOTA－Reverse方向最好。
螢光染色結果可發現間斷式刺激Parallel組可對細胞產生方向性的牽引，具有細長狀的偽足；連續式刺激Parallel組可明看到action stress fiber較短，整體細胞形狀較偏向圓形。

關鍵字

細胞遷移 ； 纖維母細胞 ； 磁場 ； 奈米材料 ； Gd-DOTA

並列摘要

A trend toward cell tracking by magnetic nanomaterials is growing during these years. However, no study was found on the cell migration when the cells were cultured with magnetic materials and exposure under magnetic field. In this study the effects of added gadolinium (Gd) and magnetic fields caused by magnetic resonance imaging (MRI) machine were evaluated on cells migration.
L929 fibroblasts cell were cultured in H-DMEM and supplemented with 10% fetal bovine serum. After cells attached, 5% Gd-DOTA was added to the cultured medium. Twenty-four hours later, cells were exposed under magnetic fields for intermittent type and continuous type. Magnetic fields were generated by a MRI machine. The cell migration was recorded by a CCD camera and analysis in Image-Pro software.
In MTT, NO and LDH assay, which means that Gd-DOTA concentration less than 15% has no cytotoxic effects on fibroblasts. In the cell migration trial, the parallel magnetic fields without Gd-DOTA ( Reverse) performed the highest value among all groups.
The parallel magnetic fields group displayed consistent alignment orientation between cultured cells for intermittent stimulates by the displayed in the immunofluorescence.

並列關鍵字

cell migration ； fibroblast ； magnetic field ； Gd-DOTA ； nanomaterial

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