白細胞間素4，白細胞間素6，白細胞間素10之基因多形體與急性和慢性兒童特發性血小板缺乏性紫斑症關聯性之研究

Interleukin 4, Interleukin 6 and Interleukin 10 Polymorphisms in hildren with Acute and Chronic Immune Thrombocytopenic Purpura

彭慶添(Ching-Tien Peng)

指導教授 ： 藍守仁

亞洲大學/健康學院/健康管理研究所/碩士(2005年)

摘要

細胞素(cytokines)在兒童特性發紫斑症有重要的角色，我們在50位急性兒童特性發紫斑症兒童，30位慢性特性發紫斑症和100位健康兒童，以分子醫學單核苷多形體基因(SNP)的方法，做白細胞間素IL-4 intron3，白細胞間素IL-6(-572G/C)，白細胞間素IL-10(-627C/A)的基因多形體(polymorphism)研究分析，結果發現在慢性特性發紫斑症和健康兒童中，IL-4 intron3的RP1/RP2基因型(genotype)的比例上有顯著的差異性 (13.3% versus 33.0%, p=0.04, odds ratio= 0.3, 95% CI=0.1-1.0)，在 RP2對偶基因的頻率(allelic frequency)也有顯著的差異性 (6.7% versus 19.5%, p=0.03, odds ratio= 1.8, 95% CI=1.1-3.2); 且IL-10(-627C/A)的A/C基因型比例亦有顯著的差異性 (13.3% versus 44.0%, p=0.01, odds ratio= 0.2, 95% CI=0.1-0.7)，但在急性特性發紫斑症和健康兒童中，IL-4 intron 3，IL-6(-572G/C)，IL-10(-627C/A)的基因多形體無顯著的差異性。這研究發現白細胞間素IL-4 intron 3和IL-10(-627C/A)的基因多形體在兒童慢性特性發紫斑症的病理機轉有重要的相關和影響。

關鍵字

白細胞間素 ； 紫斑症

並列摘要

Inflammatory cytokines are essential to the regulation of immune thrombocytopenic purpura (ITP). We investigated intron 3 variable number of tandem repeats of interleukin (IL)-4, IL-6 (-572 G/C), and IL-10 (-627 C/A) polymorphisms in 50 children with acute ITP, 30 children with chronic TIP, and 100 healthy individuals by polymerase chain reaction-based restriction analysis. There were significant differences in RP1/RP2 genotype proportion (13.3% versus 33.0%, p=0.04, odds ratio= 0.3, 95% CI=0.1-1.0) and RP2 allelic frequency (6.7% versus 19.5%, p=0.03, odds ratio= 1.8, 95% CI=1.1-3.2) of IL-4 intron 3 and A/C genotype proportion (13.3% versus 44.0%, p=0.01, odds ratio= 0.2, 95% CI=0.1-0.7) of IL-10 (-627) between the childhood chronic ITP and controls, while no significant association was detected for the other polymorphisms of IL-4 intron 3, IL-6 (-572), and IL-10 (-627) among acute ITP, chronic ITP, and controls. This finding suggest IL-4 intron 3 and IL (-627) polymorphisms may be implicated the pathogenesis of ITP or may be responsible for modulating the immune response in the heterogeneous immune disease.

並列關鍵字

白細胞間素 ； 紫斑症

參考文獻

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