以牛樟芝餵食小鼠亞急性與亞慢性毒性試驗

Subacute and Subchronic Toxicological Assessment of Antrodia Cinnamomea in Mice

吳銘芳(Ming-Fang Wu) ； 黃幸妮(Hsing-Ni Huang) ； 吳龍源(Lung-Yuan Wu) ； 周建聖(Jason Chou) ； 葉明陽(Ming-Yang Yeh) ； 呂旭峰(Hsu-Feng Lu)

《傳統醫學雜誌》 23卷1期 (2012/10) Pp. 11-22

https://doi.org/10.30038/JTM.201210.0002

摘要

在台灣，非處方之中草藥常被用來治療腹痛、高血壓與肝腫瘤，並逐漸被全世界所採用。在東方世界，這些中草藥之中又以牛樟芝爲最有名的調理聖品，因此提供毒理檢查已確定食品安全性有其必要。本硏究建立小鼠（BALB／c mice)口服28天亞急性與90天亞慢性毒性分析模式，在每組十隻老鼠且雌雄分別下，以16.7、833.3與1666.7毫克／公斤／天三種劑量連續28天或90天口服餵食，觀察其亞急性或亞慢性毒性反應。各組老鼠在實驗最後仍皆存活，且實驗組老鼠之血液與生化檢驗結果皆與對照組無統計學差異。因此，從此實驗結果可瞭解到未觀察到毒性的劑量至少爲1666.7 mg／kg／天。

並列摘要

Non-prescriptional use of medicinal herbs is highly prevalent to treat abdominal pain, hypertension and hepatocellular carcinoma in Taiwan and increasing worldwide. Among them, Antrodia cinnamomea was the most famous modalities being consumed especially among Oriental. Since the use of Antrodia cinnamomea in herbal medicines is increased, toxicity studies are warranted to confirm the safe use of Antrodia cinnamomea. Based on the 28-day subacute and 90-day subchronic oral toxicity studies of Antrodia cinnamomea extract in male and female BALB/c mice, the extract at doses of 16.7, 833.3, and 1666.7mg/kg/day were administered by oral gavage to groups of mice (10 mice/dose) for 28 or 90 consecutive days individually and sterile water was used as control. All animals survived to the end of the study. No significant differences were found in hematology and serum biochemistry parameters among the control and treatment groups. These results suggest that the no-observed-adverse-effect level (NOAEL) of the extract for male and female mice is considered to be 1666.7 mg/kg/day.