- 期刊
Fatty Acid Composition of Copepods Fed with Different Diets
餵食不同食物對於橈足類脂肪酸組成的影響
摘要
以足量之植物性餌料（等鞭金藻和周氏扁藻）和動物性食物（輪蟲益、文蛤粉、鰻粉）分別餵食兩種南台灣養殖池中常見之橈足類（短角異劍水蚤及模糊許水蚤），實驗結果發現只以動物性食物來培育的兩種橈足類實驗組皆呈現族群量受到抑制的現象；反之，只以植物性藻類餵食的兩種橈足類則族群量皆增殖迅速。在不同溫度(15、20、25、30℃)下培養之植物性藻類等鞭金藻的脂肪酸組成分析沒有顯著的差異出現。餵食兩種植物性藻類對兩種橈足類的脂肪酸組成分析與微藻做比對時發現，餵食等鞭金藻時，短角異劍水蚤及模糊許水蚤的高度不飽和脂肪酸EPA含量與並沒有差異，但DHA則分別增加了955%及755%。餵食周氏扁藻時，模糊許水蚤的EPA含量與並沒有差異，但短角異劍水蚤減少5.21%；DHA則分別增加了476%及291%。
並列摘要
Research on the dietary requirements of marine fish larvae has evolved from considerations of optimal dietary levels of n-3 highly unsaturated fatty acids (HUFA) to considerations of optimal dietary ratios of the two principal HUFAs, 22:6 n-3 (DHA) and 20:5 n-3 (EPA), and optimal dietary levels and ratios of those essential fatty acids. In this study, we found the microalgae Isochrysis galbana have relatively high concentrations of 18-carbon, 16-carbon polyunsaturated fatty acid (PUFA), and especially DHA. The DHA/EPA ratio is approximately 27:1. Microalgae Tetraselmis chuii has high concentrations of 18-carbon, 16-carbon PUFA and EPA. The DHA/EPA ratio is approximately 6.4:1. Two species of copepods, Apocyclops royi and Pseudodiaptomus annandalei, which arc commonly found as live food used in fish or shrimp culture ponds in southern Taiwan. Both copepod can not survive on feeding alone on artificial animal protein (artificial fermented liquid, powdered clam feed. powdered eel feed), but can grow abundantly when they were fed with suitable phytoplankton with sufficient quantities of either I. galbana or T chuii. When fed with I. galbana, the EPA content of A. royi and P. annandalei were similar to the alga, but DHA increased by 9.55% and 7.55%, respectively. When fed with T. chuii, the EPA content of P. annandalei was similar to the alga, but A. royi decreased by 5.21%. However Dha, increased by 4.76% and 2.91%, respectively. The DHA/EPA ratio of copepod fed with either I. galbana or T. chuii are 61.4:1, 7.9:1, 45.0:1 and 0.6:1, respectively.