Forty women who underwent amniocentesis were administered three self-development questionnaires to explore their knowledge, concerns toward amniocentesis by genetic counseling. Subjects' knowledge about amniocentesis improved after pre-amniocentesis counseling and maintained till the results of the procedure were communicated to the women. The concern scores of subjects were significantly reduced after counseling although the items that they concerned were increased. The major concerns about amniocentesis prior to the counseling were fear of fetal injury, abnormal result of amniocentesis, miscarriage and the during the procedures performed. However, after the counseling they changed to fear of fetal injury and the results of amniocentesis. From the correlation analysis, The subjects' knowledge toward amniocentesis was related to concern level prior to counseling. The findings of this study have important implications for the delivery of pre-amniocentesis counseling for clinical practice.