- 期刊
以高張性鹽溶液激發鼻黏膜產生P物質之反應
Substance P Release Evoked by Hypertonic Saline on Nasal Mucosa
摘要
背景：非特定性的刺激如組織胺、冷空氣、高張性鹽溶液等會激發呼吸道黏膜之炎症反應，例如高張性鹽溶液會引起眼睛的神經性炎症和呼吸道的咳嗽，分泌物增多等反應，其致病機轉至今仍未清楚，高張性鹽溶液可能刺激鼻黏膜感覺神經促其分泌神經胜P物質，引起續發之神經性炎症反應如腺體的分泌增加，血管的滲透性增加及黏膜下之水腫等。但至今仍無報告說明感覺神性胜反應於高張性鹽溶液刺激鼻黏膜後的情形。本實驗的目的即評估對於不同濃度高張性鹽溶液的鼻黏膜激發是否釋出不等量的感覺神經胜P物質。 方法：本實驗選擇10位無鼻黏膜疾病的病人，以不同濃度的高張性鹽溶液刺激右側黏膜後，分別收集兩側之鼻分泌液，儲於-70度備用。再以放射性免疫分析法檢測其中感覺神經胜P物質的濃度。 結果：受刺激側之鼻分泌液中感覺神經胜的含量會依高張性鹽溶液之濃度升高而增加，而對側無反應性增加。 結論：呼吸道黏膜對於高張性鹽溶液的刺激會經由感覺神經釋放P物質，引起黏膜的神經性炎症反應。
並列摘要
Background: Non-specific stimuli such as histamine, cold air, and hypertonic saline will induce mucosal inflammation of the respiratory tract. Among these, hypertonic saline may also cause ophthalmic neurogenic inflammation, cough, and hypersecretion though its pathogensis is still unclear. Hypertonic saline may induce the release of a sensory neuropeptide-substance P from the nociceptive nerves, causing subsequent neurogenic inflammation, such as hypersecretion, vascular hyperpermebility and submucosal edema. There has been no report involving sensory neuropeptide-substance P release induced by hypertonic saline in human nasal mucosa. Thus, we evaluated the substance P response in the nasal secretion after the stimulation of various concentrations of hypertonic saline. Materials and Methods: The right nasal cavity of 10 volunteers without any nasal disease was stimulated by various concentrations of hypertonic saline. Then the nasal secretion of both nasal cavities were individually collected and immediately stored at -70°C. The concentration of substance P of the secretion were measured using radiommunoassay. Results: There was in increasing trend of substance P concentration in nasal secretion, correlating to the concentration of hypertonic saline in the stimulated nasal cavity. However, there was no significant change in the opposite site. Conclusion: The hypertonic saline may induce neurogenic inflammation of the respiratory mucosa through the substance P release from nociceptive fibers.