Gender assignment is one of the most important decisions for intersex patients. An appropriate genitoplasty may be necessary for a male or female assignment in intersex patients. Feminizing genitoplasty was performed in one patient with 17-hydroxysteroid dehydrogenase deficiency. Genitoplasty in combination with gonadectomy was performed successfully in these two patients. Reconstructive operation should include restoration of labia minora, reductive surgery of clitoris and labia majora, flap plastic surgery of vagina. Injury to urethra and rectum should be carefully avoided. Feminizing genitoplasty should be performed for an ambiguous genitalia in the intersex patient following a female gender assignment.