ARS與BPR已在先前研究證實對霧狀活性蛋白質(Haze-active Protein, HAP)有高度吸附性,可應用在定量溶液中或飲料中之HAP。本研究進一步針對ARS對BPR在飲料模擬系統(model beer)下,與HAP吸附之機制進行分析與比較,本研究結果發現:【1】由於ARS與BPR在結構上,和易與HAP吸附的酚(polyhenol)類似,在ARS與Methyl Gallate (MG)的濁度(turbidity)實驗發現,ARS與MG同時存在時,兩者可能是競爭在HAP(model HAP:gliadin)上相同的結合位置(binding sites);而ARS與Tannin(TA)同時存在時,兩者亦可能是競爭在HAP上相同的結合位置。同樣地,BPR與MG或BPR與TA同時存在時,兩者皆可能是競爭在HAP (gliadin)上相同的結合位置。【2】ARS與BPR兩者在gliadin 100-600mg/L的範圍內,吸光值變化與濃度間線性度良好,R2(R square)值分別為0.9689與0.9922。【3】並驗證ARS與BPR在0-600mg/Lgliadin濃度範圍,吸光值變化與濁度值相同關性(Corelationship, R值)良好,R值分別是與0.9934與0.9848。然而先前研究指出,在一些飲料中含有UV區具吸光性的物質,故使用ARS染劑吸附法定量HAP時,建議先移除這一些干擾物以提高精準度。
Alizarin Red S (ARS) and Bromopyrogallol Red (BPR) have been utilized to estimate the haze-active protein (HAP) in beverages or test solutions. In this study, mechanisms of ARS, BPR dye binding methods for estimation of HAP in model system are conducted and studied. The results indicated that (1) the binding sites on HAP for ARS or BPR are probably the same as those for single-end polypheol MG or haze-active polypheol tannin. The dyes and polyhenols probably compete for the same sites in a HA protein. (2) The R^2 (R square) representing linear relationships between protein concentration and absorbance changes by dye-protein binding is 0.9689 and 0.9922, respectively. (3) The R value of corelationship between haze induction (TA plus gliadin) and absorbance change by dye-protein binding is 0.9934 and 0.9848, respectively. Previous studies indicate that some substances in beer also absorb light in the UV region. It is suggested to remove the UV interfering materials before ARS assay.
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