Effect of Various Pulpotomy Dressing Materials on the Viability of L929 Cells

The purpose of the present study was to evaluate the compatibility of mouse periodontal fibroblast (L929) cells with different formulas of pulpotomy dressing materials. The pulpotomy preparations were differentiated into group 1: zinc oxide powder, eugenol, and formocresol; group 2: zinc oxide powder, eugenol, and glutaraldehyde; group 3: zinc oxide powder, eugenol, and ferric sulfate; group 4: calcium hydroxide, distilled water, and formocresol; group 5: calcium hydroxide, distilled water, and glutaraldehyde; and group 6: calcium hydroxide, distilled water, and ferric sulfate. All mixed materials were dissolved in medium and diluted to 10, 20, 40,and 80 μl/ml concentrations, A cell colorimetric assay (MTT) was used to detect the viability of L929 cells. Results were compared using 1-way analysis of variance (ANOVA). Differences in treatment means were analyzed using Student-Newman-Keul's test and were considered significant at p＜0.05. Survival rates of treated L929 cells showed statistical differences as the concentrations of the pulpotomy materials increased (p＜0.05). The highest survival rates were found in groups 3, 5, and 6. It is recommended that low-toxicity formulas (such as those containing zinc oxide powder, eugenol, and ferric sulfate; calcium hydroxide, distilled water, and glutaraldehyde; or calcium hydroxide, distilled water, and ferric sulfate) be used clinically as pulpotomy dressing materials.

並列關鍵字

pulpotomy ； biocompatibility ； zinc oxide powder ； glutaraldehyde ； calcium hydroxide formocresol

國際替代計量

全文下載

主題瀏覽